PerCP/Cyanine5.5 anti-mouse LAP (TGF-β1) Anti
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse LAP (TGF-β1) Anti
- 产品类别：
- 抗体
- 产品编号：
- 141409
- 产品应用：
- 141409
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mouse Tgfb1-transduced P3U1 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Clone TW7-16B4 has been reported to not cross-react with bovine LAP.2 Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.2 TW7-16B4 recognizes recombinant LAP, latent TGF-β, and pro-TGF-β.
Additional reported applications (for relevant formats) include: Western blotting1 and immunoprecipitation1.?
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Oida T, et al. 2010. PLoS One 5:e15523. (FC, IP, WB)
- Oida T, et al. 2011. PLoS One 6:e18365. (Neut)
- Product Citations
-
- Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
- Koronyo-Hamaoui M, et al. 2020. Brain. 143:336. PubMed
- Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
- RRID
- AB_2561591 (BioLegend Cat. No. 141409) AB_2561592 (BioLegend Cat. No. 141410)
- Structure
- Dimmers of latency-associated peptide non-covalently associated with dimmers of mature TGF-β
- Distribution
- Many cell types, highly expressed on activated Tregs and platelets
- Function
- TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response
- Ligand/Receptor
- TGF-β receptors
- Cell Type
- Tregs, Platelets, Dendritic cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Oida T, et al. 2010. PLoS One 5:e15523.
2. Tran D, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.
3. Ochi H, et al. 2006. Nat. Med. 12:627.
4. Oida T, et al. 2003. J. Immunol. 170:2516.
5. Nakamura K. 2001. J. Exp. Med. 194:629.
6. Miyazono K, et al. 1993. Growth Factors 8:11.
- Gene ID
- 21803 View all products for this Gene ID
- UniProt
- View information about LAP on UniProt.org
