Brilliant Violet 421™ anti-mouse LAP (TGF-&b
- 产品名称：
- Brilliant Violet 421™ anti-mouse LAP (TGF-&b
- 产品类别：
- 抗体
- 产品编号：
- 141407
- 产品应用：
- 141407
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mouse Tgfb1-transduced P3U1 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- ?g size: 0.2 mg/mL?L size: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Clone TW7-16B4 has been reported to not cross-react with bovine LAP.2 Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.2 TW7-16B4 recognizes recombinant LAP, latent TGF-β, and pro-TGF-β.
Additional reported applications (for relevant formats) include: Western blotting1 and immunoprecipitation1.?
- Application References
(PubMed link indicates BioLegend citation) -
- Oida T, et al. 2010. PLoS One 5:e15523. (FC, IP, WB)
- Oida T, et al. 2011. PLoS One 6:e18365. (Neut)
- Product Citations
-
- Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
- Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
- Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
- Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
- De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
- Bachy S, et al. 2022. iScience. 25:103758. PubMed
- Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
- Zheng G, et al. 2017. Respir Res. 10.1186/s12931-017-0599-5. PubMed
- Prakash MD, et al. 2021. PLoS One. 16:e0247492. PubMed
- Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
- Culina S, et al. 2015. Diabetes. 64: 3532 - 3542. PubMed
- L M, et al. 2016. Brain. 139: 1939-1957. PubMed
- Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
- RRID
- AB_2561580 (BioLegend Cat. No. 141407) AB_2650898 (BioLegend Cat. No. 141408)
- Structure
- Dimmers of latency-associated peptide non-covalently associated with dimmers of mature TGF-β
- Distribution
- Many cell types, highly expressed on activated Tregs and platelets
- Function
- TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response
- Ligand/Receptor
- TGF-β receptors
- Cell Type
- Tregs, Platelets, Dendritic cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Oida T, et al. 2010. PLoS One 5:e15523.
2. Tran D, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.
3. Ochi H, et al. 2006. Nat. Med. 12:627.
4. Oida T, et al. 2003. J. Immunol. 170:2516.
5. Nakamura K. 2001. J. Exp. Med. 194:629.
6. Miyazono K, et al. 1993. Growth Factors 8:11.
- Gene ID
- 21803 View all products for this Gene ID
- UniProt
- View information about LAP on UniProt.org
