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首页 > 抗体蛋白 > 抗体
Purified anti-Tubulin β 3 (TUBB3) Antibody (P
产品名称：
Purified anti-Tubulin β 3 (TUBB3) Antibody (P
产品类别：
抗体
产品编号：
801213
产品应用：
801213
[价格]
规格 价格 库存
25µL ￥ 1484 1

产品详情

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against microtubules derived from rat brain.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P -?Quality tested
WB, ICC -?Verified
FC -?Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 ?g/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 ?g/ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: flow cytometry4, immunofluorescence microscopy1-5,7, immunohistochemistry5,7,?Western blotting8, and spatial biology (IBEX)9,10.

This antibody is well characterized and highly reactive to neuron specific Class III ?-tubulin (?III). TUJ1 does not identify ?-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues8.

Application References

(PubMed link indicates BioLegend citation)
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  9. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  10. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
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RRID
AB_2728521 (BioLegend Cat. No. 801213) AB_2313773 (BioLegend Cat. No. 801201) AB_10063408 (BioLegend Cat. No. 801202)
Disclaimer

Covered by US patents 5,675,063 and 7,429,487. Sold under license from Epitomics.

Antigen Details

Structure
Tubulin β 3 is a 450 amino acid protein with a molecular mass of ~50 kD.
Distribution

Tissue distribution: central and peripheral nervous system.
Cellular distribution: cytosol, cytoskeleton and nucleus.

Function
Tubulin β 3 is the major constituent of microtubules, and plays a critical role in proper axon guidance and maintenance.
Interaction
Alpha tubulin, kinesin and dynein.
Cell Type
Mature Neurons, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Microtubules
Antigen References

1. Zhao X,?et al.?2017. Med Sci Monit. 22: 3915.
2.?Lebok P,?et al. 2016. Oncol Lett. 11(3):1987.
3. Du J,?et al.?2015. BMC Cancer. 15:536.?PubMed
4.?Rogue DM.,?et al. 2013. Clin Exp Metastasis. 31(1): 101.
5.?Ploussard G,?et al.?2010. Cancer Res. 70(22):9253.?PubMed

Gene ID
10381 View all products for this Gene ID
UniProt
View information about Tubulin beta-3 on UniProt.org

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