PE anti-Tubulin β 3 (TUBB3) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1876	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

This antibody was raised against microtubules derived from rat brain.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: flow cytometry⁴, immunofluorescence microscopy^1-5,7, immunohistochemistry^5,7,?Western blotting⁸, and spatial biology (IBEX)^9,10.

This antibody is well characterized and highly reactive to neuron specific Class III ?-tubulin (?III). TUJ1 does not identify ?-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues^8.

Application References (PubMed link indicates BioLegend citation)

Nishimura K, et al. 2017. PLoS One. 12(1): e0170568. (ICC)
Jongbloets J, et al. 2017. Nat Commun. 8: 14666. (ICC) PubMed
Liu W.J, et al. 2015. Eur J Histochem. 59(1): 2464. (ICC) PubMed
Chintalapudi SR, et al. 2016. Front Aging Neursci. 8:93. (FC, ICC) PubMed
Ambasudhan R, et al. 2011. Cell Stem Cell. 9(2):113. (IHC, ICC)
Hu X., et al. 2006. Nature Neuroscicene. 9(12):1520. (WB) PubMed
Zechner D., et al. 2003. Develop Biology. 258(2):406. (ICC, IHC)
Lee MK, et al. 1990. Proc. Natl. Acad. Sci. USA 18:7195. (WB)
Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

RRID

AB_2892489 (BioLegend Cat. No. 801221) AB_2892489 (BioLegend Cat. No. 801222)

Antigen Details

Structure: Tubulin β 3 is a 450 amino acid protein with a molecular mass of ~50 kD.
Distribution: Tissue distribution: central and peripheral nervous system.
Cellular distribution: cytosol, cytoskeleton and nucleus.
Function: Tubulin β 3 is the major constituent of microtubules, and plays a critical role in proper axon guidance and maintenance.
Interaction: Alpha tubulin, kinesin and dynein.
Cell Type: Mature Neurons, Neural Stem Cells
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Microtubules
Antigen References: 1. Zhao X,?et al.?2017. Med Sci Monit. 22: 3915.
2.?Lebok P,?et al. 2016. Oncol Lett. 11(3):1987.
3. Du J,?et al.?2015. BMC Cancer. 15:536.?PubMed
4.?Rogue DM.,?et al. 2013. Clin Exp Metastasis. 31(1): 101.
5.?Ploussard G,?et al.?2010. Cancer Res. 70(22):9253.?PubMed
Gene ID: 10381 View all products for this Gene ID
UniProt: View information about Tubulin beta-3 on UniProt.org

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