Alexa Fluor® 647 anti-Tubulin β 3 (TUBB3)-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1974	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

This antibody was raised against microtubules derived from rat brain.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICC -?Quality tested
ICFC, 3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤5.0 ?g per million cells. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: flow cytometry⁴, immunofluorescence microscopy^1-5,7, immunohistochemistry^5,7, and Western blotting⁸.

This antibody is well characterized and highly reactive to neuron specific Class III ?-tubulin (?III). TUJ1 does not identify ?-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues^8.

Application References (PubMed link indicates BioLegend citation)

Nishimura K, et al. 2017. PLoS One. 12(1): e0170568. (ICC)
Jongbloets J, et al. 2017. Nat Commun. 8: 14666. (ICC) PubMed
Liu W.J, et al. 2015. Eur J Histochem. 59(1): 2464. (ICC) PubMed
Chintalapudi SR, et al. 2016. Front Aging Neursci. 8:93. (FC, ICC) PubMed
Ambasudhan R, et al. 2011. Cell Stem Cell. 9(2):113. (IHC, ICC)
Hu X., et al. 2006. Nature Neuroscicene. 9(12):1520. (WB) PubMed
Zechner D., et al. 2003. Develop Biology. 258(2):406. (ICC, IHC)
Lee MK, et al. 1990. Proc. Natl. Acad. Sci. USA 18:7195. (WB)

Product Citations

Arsić A, et al. 2020. Sci Rep. 10:6441. PubMed
Holman HA, et al. 2019. Front Cell Neurosci. 13:186. PubMed
Funato K, et al. 2021. Cell Stem Cell. 28(5):894-905.e7. PubMed
Bloom ML, et al. 2020. Chemical Senses. 45(5):333-346. PubMed

RRID

AB_2686930 (BioLegend Cat. No. 801209) AB_2686931 (BioLegend Cat. No. 801210)

Antigen Details

Structure: Tubulin β 3 is a 450 amino acid protein with a molecular mass of ~50 kD.
Distribution: Tissue distribution: central and peripheral nervous system.
Cellular distribution: cytosol, cytoskeleton and nucleus.
Function: Tubulin β 3 is the major constituent of microtubules, and plays a critical role in proper axon guidance and maintenance.
Interaction: Alpha tubulin, kinesin and dynein.
Cell Type: Mature Neurons, Neural Stem Cells
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Microtubules
Antigen References: 1. Zhao X,?et al.?2017. Med Sci Monit. 22: 3915.
2.?Lebok P,?et al. 2016. Oncol Lett. 11(3):1987.
3. Du J,?et al.?2015. BMC Cancer. 15:536.?PubMed
4.?Rogue DM.,?et al. 2013. Clin Exp Metastasis. 31(1): 101.
5.?Ploussard G,?et al.?2010. Cancer Res. 70(22):9253.?PubMed
Gene ID: 10381 View all products for this Gene ID
UniProt: View information about Tubulin beta-3 on UniProt.org

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