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Alexa Fluor® 488 anti-Tubulin β 3 (TUBB3)
产品名称：
Alexa Fluor® 488 anti-Tubulin β 3 (TUBB3)
产品类别：
抗体
产品编号：
801203
产品应用：
801203
[价格]
规格 价格 库存
100µL ￥ 4928 1

产品详情

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against microtubules derived from rat brain.
Formulation
Alexa Fluor? Labeled Antibody (in PBS + 50% glycerol + 0.03% thimerosal).
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-P, ICFC, 3D IHC - Verified
SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution of 1:500 is recommended. For immunohistochemistry, a concentration range of 1.0 - 5.0 ?g/ml is suggested.?For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: flow cytometry4, immunofluorescence microscopy1-5,7, immunohistochemistry5,7,?Western blotting8, and spatial biology (IBEX)9,10.

This antibody is well characterized and highly reactive to neuron specific Class III ?-tubulin (?III). TUJ1 does not identify ?-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues8.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Nishimura K, et al. 2017. PLoS One. 12(1): e0170568. (ICC)
  2. Jongbloets J, et al. 2017. Nat Commun. 8: 14666. (ICC) PubMed
  3. Liu W.J, et al. 2015. Eur J Histochem. 59(1): 2464. (ICC) PubMed
  4. Chintalapudi SR, et al. 2016. Front Aging Neursci. 8:93. (FC, ICC) PubMed
  5. Ambasudhan R, et al. 2011. Cell Stem Cell. 9(2):113. (IHC, ICC)
  6. Hu X., et al. 2006. Nature Neuroscicene. 9(12):1520. (WB) PubMed
  7. Zechner D., et al. 2003. Develop Biology. 258(2):406. (ICC, IHC)
  8. Lee MK, et al. 1990. Proc. Natl. Acad. Sci. USA 18:7195. (WB)
  9. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  10. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Lu R, et al. 2021. Int J Mol Sci. 22:00. PubMed
  2. Giacomini C, et al. 2016. Mol Biol Cell. 27: 35 - 47. PubMed
  3. McCurdy EP et al. 2019. Cell Rep. 29(2):363-377 . PubMed
  4. Babetto E, et al. 2020. Nat Neurosci. 23:1215. PubMed
  5. Rockt?schel P, et al. 2019. Epilepsy Behav. 101:106581. PubMed
  6. Guzmán M, et al. 2020. Immunology. 161:148. PubMed
  7. Kim Y, et al. 2016. Sci Rep. 6: 23799. PubMed
  8. Inlender T, et al. 2021. Scientific Reports. 11(1):12796. PubMed
  9. Arsić A, et al. 2020. Sci Rep. 10:6441. PubMed
  10. Wang S, et al. 2022. PLoS Pathog. 18:e1010281. PubMed
  11. Stachtea X, et al. 2015. PLoS One. 10: e0140279. PubMed
  12. Larhammar M et al. 2017. eLife. 6 pii: e20725. PubMed
  13. Jalilian E, et al. 2022. Stem Cell Res Ther. 13:425. PubMed
  14. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  15. Martínez JC et al. 2019. Neuron. 104(5):931-946 . PubMed
  16. Geisler S, et al. 2019. JCI Insight. 4:e129920. PubMed
  17. ?iak J, et al. 2020. EMBO Rep. e48512:21. PubMed
  18. Chung KM, et al. 2022. Cell Rep. 41:111488. PubMed
RRID
AB_2564757 (BioLegend Cat. No. 801203)
Disclaimer

Covered by US patents 5,675,063 and 7,429,487. Sold under license from Epitomics.

Antigen Details

Structure
Tubulin β 3 is a 450 amino acid protein with a molecular mass of ~50 kD.
Distribution

Tissue distribution: central and peripheral nervous system.
Cellular distribution: cytosol, cytoskeleton and nucleus.

Function
Tubulin β 3 is the major constituent of microtubules, and plays a critical role in proper axon guidance and maintenance.
Interaction
Alpha tubulin, kinesin and dynein.
Cell Type
Mature Neurons, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Microtubules
Antigen References

1. Zhao X,?et al.?2017. Med Sci Monit. 22: 3915.
2.?Lebok P,?et al. 2016. Oncol Lett. 11(3):1987.
3. Du J,?et al.?2015. BMC Cancer. 15:536.?PubMed
4.?Rogue DM.,?et al. 2013. Clin Exp Metastasis. 31(1): 101.
5.?Ploussard G,?et al.?2010. Cancer Res. 70(22):9253.?PubMed

Gene ID
10381 View all products for this Gene ID
UniProt
View information about Tubulin beta-3 on UniProt.org

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