Purified anti-β-Tubulin Antibody (Previously
- 产品名称：
- Purified anti-β-Tubulin Antibody (Previously
- 产品类别：
- 抗体
- 产品编号：
- 903401
- 产品应用：
- 903401
- Verified Reactivity
- Bovine, Rat, Chinese Hamster, Sea Urchin, Chlamydamonas, Drosophila
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was generated against taxol-assembled microtubules.
- Formulation
- Phosphate-buffered solution + 0.03% Thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
WB, ICC, IHC
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting.
The optimal working dilution should be determined for each specific assay condition.
??WB:?1 ?g/mL?(1:1000)
??IF: 1,000
- Application Notes
Reactivity to Drosophila was only verified with the purified format.
This antibody is effective in immunoblotting and immunostaining.
*Predicted MW = 55 kD
The TU27 clone is broadly reactive to b-tubulin subtypes and binds outside of the C-terminal isotype domains, but does not cross react with alpha-tubulin by immunoblotting. It recognizes a highly conserved epitope and reacts with ?-tubulin found in vertebrate as well as invertebrate tissues.
- Additional Product Notes
For Western blotting, the suggested use of this reagent is 1.0 - 10 ?g/mL in Drosophila and 1.0 ?g/mL in other species.
- Application References
(PubMed link indicates BioLegend citation) -
- MK Lee, et al. 1990. Cell Motil. Cytoskel. 17:118.
- JE Alexander, et al. 1991. Proc Natl Acad Sci USA. 88:4685.
- J Lisztwan, et al. 1998. EMBO J. 17:368.
- Fujita T, et al. 2015. PLoS One. 10:e0116579. (WB) PubMed
- Ai M, et al. 2013. J. Neurosci. 33:10741. (WB) PubMed
- Product Citations
-
- Venkatesan A, et al. 2019. Int J Mol Sci. 20:4. PubMed
- Lence T, et al. 2016. Nature. 540:242-247. PubMed
- Mitra S, et al. 2021. Dev Biol. 478:205. PubMed
- Heine D, et al. 2016. Mycologia. 108: 581 - 589. PubMed
- Courgeon M, et al. 2018. J Cell Sci. 131:jcs220251. PubMed
- Banerjee SJ, et al. 2021. PLoS One. 16:e0256738. PubMed
- Ho CH, et al. 2022. EMBO Rep. 23:e53231. PubMed
- Ai M, et al. 2013. J Neurosci. 33:10741-10749. PubMed
- Fujita T, et al. 2015. PLoS One. 10:e0116579. PubMed
- Astigarraga S, et al. 2018. Development. 145:. PubMed
- Swiatlowska P, et al. 2020. Nanoscale. 12:16315. PubMed
- de Jesus AA, et al. 2019. J Allergy Clin Immunol. 143:1939. PubMed
- Sharabi A, et al. 2019. JCI Insight. 4:e126294. PubMed
- Sah M, et al. 2020. Neurobiology of Disease. 78.45694444. PubMed
- Vernia S et al. 2016. eLife. 5 pii: e17672. PubMed
- Bawankar P, et al. 2021. Nat Commun. 12:3778. PubMed
- Knuckles P, et al. 2018. Genes Dev. 29:13. PubMed
- Church TW, et al. 2021. Elife. 10:. PubMed
- Kwon OS, et al. 2021. Nat Commun. 12:1351. PubMed
- Wan J, et al. 2016. Brain. 139: 2877 - 2890. PubMed
- RRID
- AB_2565030 (BioLegend Cat. No. 903401)
