Ultra-LEAF™ Purified anti-human CD58 (LFA-3)-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3780	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human cytolytic T cells

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, IHC, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications include: immunoprecipitation¹, inhibition of cytolytic activity¹, augment of IL-1 release by TE cells²

Application References (PubMed link indicates BioLegend citation)

Sanchez-Madrid F, et al. 1982. Proc. Nati Acad. Sci. USA. 79:7489
Le PT, et al. 1990. J. Immunol. 144:4541

RRID

AB_2832648 (BioLegend Cat. No. 330923) AB_2832649 (BioLegend Cat. No. 330924)

Antigen Details

Structure: Immunoglobulin superfamily member, 45-70 kD cell surface protein. Alternative splicing gives rise to transmembrane and glycosylphosphatidylinositol (GPI)-anchored form on cell surface.
Distribution: Expressed on both hematopoietic and non-hematopoietic cells including B cells, T cells, monocytes, erythrocytes, endothelial cells, epithelial cells, and fibroblasts. High levels are observed on memory T cells and dendritic cells.
Function: Mediates adhesion between various cell types (target cells and cytotoxic cells, APC and T cells, thymocytes and thymic epithelia).
Ligand/Receptor: CD2
Cell Type: B cells, Dendritic cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Springer TA, et al. 1987. Annu. Rev. Immunol. 5:223.
2. Dustin ML, et al. 1987. Nature 329:846.
3. Arulanandam AR, et al. 1994. J. Exp. Med. 180:1861.
4. Sanders ME, et al. 1988. J. Immunol. 140:1401.
Gene ID: 965 View all products for this Gene ID
UniProt: View information about CD58 on UniProt.org

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