PE/Cyanine5 anti-human CD58 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2058	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human cytolytic T cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications include: immunoprecipitation¹, inhibition of cytolytic activity¹, augment of IL-1 release by TE cells²

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Sanchez-Madrid F, et al. 1982. Proc. Nati Acad. Sci. USA. 79:7489
Le PT, et al. 1990. J. Immunol. 144:4541

Product Citations

Grandclaudon M, et al. 2019. Cell. 179:432. PubMed

RRID

AB_1227576 (BioLegend Cat. No. 330909)

Antigen Details

Structure: Immunoglobulin superfamily member, 45-70 kD cell surface protein. Alternative splicing gives rise to transmembrane and glycosylphosphatidylinositol (GPI)-anchored form on cell surface.
Distribution: Expressed on both hematopoietic and non-hematopoietic cells including B cells, T cells, monocytes, erythrocytes, endothelial cells, epithelial cells, and fibroblasts. High levels are observed on memory T cells and dendritic cells.
Function: Mediates adhesion between various cell types (target cells and cytotoxic cells, APC and T cells, thymocytes and thymic epithelia).
Ligand/Receptor: CD2
Cell Type: B cells, Dendritic cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Springer TA, et al. 1987. Annu. Rev. Immunol. 5:223.
2. Dustin ML, et al. 1987. Nature 329:846.
3. Arulanandam AR, et al. 1994. J. Exp. Med. 180:1861.
4. Sanders ME, et al. 1988. J. Immunol. 140:1401.
Gene ID: 965 View all products for this Gene ID
UniProt: View information about CD58 on UniProt.org

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