PerCP/Cyanine5.5 anti-human CD49a Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-human CD49a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 328321
- 产品应用：
- 328321
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1806 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human CTL line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC -?Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.?
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications include: immunoprecipitation1,?immunohistochemical staining1?of acetone-fixed frozen tissue sections, and spatial biology (IBEX)3,4.
- Application References
(PubMed link indicates BioLegend citation) -
- Hemler ME, et al. 1984. J.Immunol. 132:3011
- Hemler ME, et al. 1985. J. Biol. Chem. 260:15246
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- RRID
- AB_2876635 (BioLegend Cat. No. 328321) AB_2876635 (BioLegend Cat. No. 328322)
- Structure
- Integrin alpha chain family, Type I membrane protein, alpha chain of heterodimeric integrin receptor, 200 kD. Associates with CD29 to form VLA-1 complex
- Distribution
-
Activated T cells, monocytes, NK cells, smooth muscle cells, neuronal cells, fibroblasts, and mesenchymal cells
- Function
- Adhesion, leukocyte migration
- Ligand/Receptor
- With integrin β1 (CD29) forms receptor for laminin-1 and collagen IV
- Cell Type
- Fibroblasts, Mesenchymal cells, Mesenchymal Stem Cells, Monocytes, NK cells, T cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss Press. p122
- Boiret N, et al. 2005. Exp. Hematol. 33:219
- Gene ID
- 3672 View all products for this Gene ID
- UniProt
- View information about CD49a on UniProt.org
