PE anti-human CD49a Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1470	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human CTL line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
SB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications include: immunoprecipitation¹,?immunohistochemical staining¹?of acetone-fixed frozen tissue sections, and spatial biology (IBEX)^3,4.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References (PubMed link indicates BioLegend citation)

Hemler ME, et al. 1984. J.Immunol. 132:3011
Hemler ME, et al. 1985. J. Biol. Chem. 260:15246
Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

Product Citations

Corleis B, et al. 2019. Cell Rep. 26:1409. PubMed
Martrus G, et al. 2017. PLoS One.. 10.1371/journal.pone.0182532. PubMed
Zhang Z, Shively J 2013. PLoS One. 8:67649. PubMed
Qualai J, et al. 2016. PLoS One. 11: 0156605. PubMed
Sandoval F, et al. 2013. Sci Transl Med. 13:172. PubMed
Mastrogiovanni M, et al. 2022. Sci Adv. 8:eabl5942. PubMed
Marquardt N, et al. 2015. J Immunol. 194:2467. PubMed
Kawamoto E, et al. 2021. Biomedicines. 9:. PubMed

RRID

AB_1236441 (BioLegend Cat. No. 328303) AB_1236407 (BioLegend Cat. No. 328304)

Antigen Details

Structure

Integrin alpha chain family, Type I membrane protein, alpha chain of heterodimeric integrin receptor, 200 kD. Associates with CD29 to form VLA-1 complex

Distribution

Activated T cells, monocytes, NK cells, smooth muscle cells, neuronal cells, fibroblasts, and mesenchymal cells

Function

Adhesion, leukocyte migration

Ligand/Receptor

With integrin β₁ (CD29) forms receptor for laminin-1 and collagen IV

Cell Type

Fibroblasts, Mesenchymal cells, Mesenchymal Stem Cells, Monocytes, NK cells, T cells

Biology Area

Immunology, Stem Cells

Molecular Family

Adhesion Molecules, CD Molecules

Antigen References

Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss Press. p122
Boiret N, et al. 2005. Exp. Hematol. 33:219

Gene ID