Ultra-LEAF™ Purified anti-human CD29 Antibod-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3780	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cow, Cynomolgus, Dog, Horse, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
ICC?- Verified
IP, IHC, Activ - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation³, immunohistochemical staining of acetone-fixed frozen tissue sections^3,5, and activation of integrin ?₁^4,7,8. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 303010). Clone TS2/16 recognizes epitope A2.¹⁰

Application References (PubMed link indicates BioLegend citation)

Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208.
Hemler ME, et al. 1984. J. Immunol. 132:3011. (IHC, IP)
Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ)
Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC)
Murga M, et al. 2005. Blood 105:1992. (FC) PubMed
Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ)
Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ)
Wesseling J, et al. 1995. J. Cell. Biol. 129:255. (Dog Reactivity)
Rubio G, et al. 2002. Cancer Immunol. Immunother. 51:130.
Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
Paebst F, et al. 2014. Cytometry A. 85(8):678-87. (Horse reactivity)

RRID

AB_2860779 (BioLegend Cat. No. 303035) AB_2860780 (BioLegend Cat. No. 303036)

Antigen Details

Structure: Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α₁-α₆), also associates with CD51 (α_V), and α₇- α₉, 130 kD
Distribution: Lymphocytes, monocytes, granulocytes (low), platelets, mast cells, fibroblasts, endothelial cells
Function: Cell-cell and cell-matrix interactions
Ligand/Receptor: VCAM-1, MAdCAM-1, ECM
Cell Type: Embryonic Stem Cells, Endothelial cells, Fibroblasts, Granulocytes, Lymphocytes, Mast cells, Mesenchymal Stem Cells, Monocytes, Platelets, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Hemler M. 1990. Annu. Rev. Immunol. 8:365.
2. Hynes R. 1992. Cell 69:11.
Gene ID: 3688 View all products for this Gene ID
UniProt: View information about CD29 on UniProt.org

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