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首页 > 抗体蛋白 > 抗体
Purified anti-human CD29 Antibody
产品名称：
Purified anti-human CD29 Antibody
产品类别：
抗体
产品编号：
303001
产品应用：
303001
[价格]
规格 价格 库存
25µg ￥ 826 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cow, Cynomolgus, Dog, Horse, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC?- Verified
IHC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per 106 cells in 100 ?L?volume or 100 ?L?of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen tissue sections3,5, and activation of integrin ?14,7,8. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 303010). Clone TS2/16 recognizes epitope A2.10

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208.
  3. Hemler ME, et al. 1984. J. Immunol. 132:3011. (IHC, IP)
  4. Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ)
  5. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC)
  6. Murga M, et al. 2005. Blood 105:1992. (FC) PubMed
  7. Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ)
  8. Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ)
  9. Wesseling J, et al. 1995. J. Cell. Biol. 129:255. (Dog Reactivity)
  10. Rubio G, et al. 2002. Cancer Immunol. Immunother. 51:130.
  11. Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
  12. Paebst F, et al. 2014. Cytometry A. 85(8):678-87. (Horse reactivity)
Product Citations
  1. Mendt M, et al. 2018. JCI Insight. 9:1523. PubMed
  2. Ceccarelli S, et al. 2022. Front Cell Dev Biol. 10:926180. PubMed
  3. Friedman D, et al. 2021. J Cell Sci. 134:. PubMed
  4. Pilbauerova N, et al. 2021. Int J Mol Sci. 22:. PubMed
  5. Sivasubramaniyan K, et al. 2015. Glycobiology. 25: 902-917. PubMed
  6. Pillozzi S, et al. 2011. Blood. 117:902. PubMed
  7. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  8. Novoseletskaya E, et al. 2020. Front Cell Dev Biol. 8:555378. PubMed
  9. Horwacik I, et al. 2017. Int J Oncol. 50:1899. PubMed
  10. Blázquez-Prunera A, et al. 2017. Stem Cell Res Ther. 10.1186/s13287-017-0552-z. PubMed
  11. Zhang X, et al. 2011. Am J Physiol Cell Physiol. 301:1017. PubMed
  12. Cho YY, et al. 2019. PLoS One. 14:e0210535. PubMed
  13. Olin A, et al. 2018. Cell. 174:1277. PubMed
  14. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  15. Henrick BM, et al. 2021. Cell. . PubMed
  16. Tanigawa K, et al. 2019. J Cell Physiol. 234:17280. PubMed
  17. Freeman SA, et al. 2020. Science. 367:301. PubMed
  18. Rogers MA, et al. 2022. Front Cardiovasc Med. 9:778919. PubMed
  19. Conway R, et al. 2006. Mol Cell Biol. 26:5310. PubMed
  20. Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed
  21. Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
RRID
AB_314317 (BioLegend Cat. No. 303001) AB_314318 (BioLegend Cat. No. 303002)

Antigen Details

Structure
Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α1-α6), also associates with CD51 (αV), and α7- α9, 130 kD
Distribution

Lymphocytes, monocytes, granulocytes (low), platelets, mast cells, fibroblasts, endothelial cells

Function
Cell-cell and cell-matrix interactions
Ligand/Receptor
VCAM-1, MAdCAM-1, ECM
Cell Type
Embryonic Stem Cells, Endothelial cells, Fibroblasts, Granulocytes, Lymphocytes, Mast cells, Mesenchymal Stem Cells, Monocytes, Platelets, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hemler M. 1990. Annu. Rev. Immunol. 8:365.
2. Hynes R. 1992. Cell 69:11.

Gene ID
3688 View all products for this Gene ID
UniProt
View information about CD29 on UniProt.org

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