Purified anti-human CD29 (Maxpar® Ready) Antib-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1974	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cow, Cynomolgus, Dog, Horse, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation³, immunohistochemical staining of acetone-fixed frozen tissue sections^3,5, and activation of integrin ?₁^4,7,8. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 303010). Clone TS2/16 recognizes epitope A2.¹⁰

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208.
Hemler ME, et al. 1984. J. Immunol. 132:3011. (IHC, IP)
Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ)
Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC)
Murga M, et al. 2005. Blood 105:1992. (FC) PubMed
Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ)
Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ)
Wesseling J, et al. 1995. J. Cell. Biol. 129:255. (Dog Reactivity)
Rubio G, et al. 2002. Cancer Immunol. Immunother. 51:130.
Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
Paebst F, et al. 2014. Cytometry A. 85(8):678-87. (Horse reactivity)

Product Citations

Hwang B, et al. 2021. Nat Methods. 18:903. PubMed

RRID

AB_2563738 (BioLegend Cat. No. 303021)

Antigen Details

Structure: Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α₁-α₆), also associates with CD51 (α_V), and α₇- α₉, 130 kD
Distribution: Lymphocytes, monocytes, granulocytes (low), platelets, mast cells, fibroblasts, endothelial cells
Function: Cell-cell and cell-matrix interactions
Ligand/Receptor: VCAM-1, MAdCAM-1, ECM
Cell Type: Embryonic Stem Cells, Endothelial cells, Fibroblasts, Granulocytes, Lymphocytes, Mast cells, Mesenchymal Stem Cells, Monocytes, Platelets, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Hemler M. 1990. Annu. Rev. Immunol. 8:365.
2. Hynes R. 1992. Cell 69:11.
Gene ID: 3688 View all products for this Gene ID
UniProt: View information about CD29 on UniProt.org

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