PE/Cyanine7 anti-human CD29 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1974	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cow, Cynomolgus, Dog, Horse, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation³, immunohistochemical staining of acetone-fixed frozen tissue sections^3,5, and activation of integrin ?₁^4,7,8. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 303010). Clone TS2/16 recognizes epitope A2.¹⁰

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208.
Hemler ME, et al. 1984. J. Immunol. 132:3011. (IHC, IP)
Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ)
Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC)
Murga M, et al. 2005. Blood 105:1992. (FC) PubMed
Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ)
Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ)
Wesseling J, et al. 1995. J. Cell. Biol. 129:255. (Dog Reactivity)
Rubio G, et al. 2002. Cancer Immunol. Immunother. 51:130.
Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
Paebst F, et al. 2014. Cytometry A. 85(8):678-87. (Horse reactivity)

Product Citations

Port JR, et al. 2020. J Virol. 94:. PubMed
Mastrogiovanni M, et al. 2022. Sci Adv. 8:eabl5942. PubMed

RRID

AB_2716097 (BioLegend Cat. No. 303025) AB_2716098 (BioLegend Cat. No. 303026)

Antigen Details

Structure: Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α₁-α₆), also associates with CD51 (α_V), and α₇- α₉, 130 kD
Distribution: Lymphocytes, monocytes, granulocytes (low), platelets, mast cells, fibroblasts, endothelial cells
Function: Cell-cell and cell-matrix interactions
Ligand/Receptor: VCAM-1, MAdCAM-1, ECM
Cell Type: Embryonic Stem Cells, Endothelial cells, Fibroblasts, Granulocytes, Lymphocytes, Mast cells, Mesenchymal Stem Cells, Monocytes, Platelets, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Hemler M. 1990. Annu. Rev. Immunol. 8:365.
2. Hynes R. 1992. Cell 69:11.
Gene ID: 3688 View all products for this Gene ID
UniProt: View information about CD29 on UniProt.org

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