Alexa Fluor® 700 anti-human CD29 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1736	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cow, Cynomolgus, Dog, Horse, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation³, immunohistochemical staining of acetone-fixed frozen tissue sections^3,5, and activation of integrin ?₁^4,7,8. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 303010). Clone TS2/16 recognizes epitope A2.¹⁰

Application References (PubMed link indicates BioLegend citation)

Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208.
Hemler ME, et al. 1984. J. Immunol. 132:3011. (IHC, IP)
Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ)
Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC)
Murga M, et al. 2005. Blood 105:1992. (FC) PubMed
Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ)
Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ)
Wesseling J, et al. 1995. J. Cell. Biol. 129:255. (Dog Reactivity)
Rubio G, et al. 2002. Cancer Immunol. Immunother. 51:130.
Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
Paebst F, et al. 2014. Cytometry A. 85(8):678-87. (Horse reactivity)

Product Citations

Pelon F, et al. 2020. Nat Commun. 0.738888889. PubMed
Kieffer Y, et al. 2020. Cancer Discov. 1.340277778. PubMed
Nguyen M et al. 2018. Cell reports. 25(13):3884-3893 . PubMed
Costa A et al. 2018. Cancer cell. 33(3):463-479 . PubMed
Grandclaudon M, et al. 2019. Cell. 179:432. PubMed
Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed
Port JR, et al. 2020. J Virol. 94:. PubMed
Magagna I, et al. 2021. Cancers (Basel). 13:. PubMed

RRID

AB_2130079 (BioLegend Cat. No. 303019) AB_2130078 (BioLegend Cat. No. 303020)

Antigen Details

Structure: Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α₁-α₆), also associates with CD51 (α_V), and α₇- α₉, 130 kD
Distribution: Lymphocytes, monocytes, granulocytes (low), platelets, mast cells, fibroblasts, endothelial cells
Function: Cell-cell and cell-matrix interactions
Ligand/Receptor: VCAM-1, MAdCAM-1, ECM
Cell Type: Embryonic Stem Cells, Endothelial cells, Fibroblasts, Granulocytes, Lymphocytes, Mast cells, Mesenchymal Stem Cells, Monocytes, Platelets, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Hemler M. 1990. Annu. Rev. Immunol. 8:365.
2. Hynes R. 1992. Cell 69:11.
Gene ID: 3688 View all products for this Gene ID
UniProt: View information about CD29 on UniProt.org

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