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Alexa Fluor® 488 anti-human CD29 Antibody
产品名称：
Alexa Fluor® 488 anti-human CD29 Antibody
产品类别：
抗体
产品编号：
303015
产品应用：
303015
[价格]
规格 价格 库存
25tests ￥ 1736 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cow, Cynomolgus, Dog, Horse, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen tissue sections3,5, and activation of integrin ?14,7,8. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 303010). Clone TS2/16 recognizes epitope A2.10

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208.
  3. Hemler ME, et al. 1984. J. Immunol. 132:3011. (IHC, IP)
  4. Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ)
  5. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC)
  6. Murga M, et al. 2005. Blood 105:1992. (FC) PubMed
  7. Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ)
  8. Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ)
  9. Wesseling J, et al. 1995. J. Cell. Biol. 129:255. (Dog Reactivity)
  10. Rubio G, et al. 2002. Cancer Immunol. Immunother. 51:130.
  11. Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
  12. Paebst F, et al. 2014. Cytometry A. 85(8):678-87. (Horse reactivity)
Product Citations
  1. Porat-Shliom N, et al. 2013. PLoS One. 25:81897. PubMed
  2. Mendt M, et al. 2018. JCI Insight. 9:1523. PubMed
  3. Buffone A, et al. 2018. J Cell Sci. 131:. PubMed
  4. Esteves C, et al. 2017. Stem Cell Res Ther. 10.1186/s13287-017-0525-2. PubMed
  5. Bitton A, et al. 2021. Cytometry A. . PubMed
  6. Morad G, et al. 2020. International Journal of Molecular Sciences. 21(11):3851. PubMed
  7. Paebst F, et al. 2016. Cytometry A. 85:678-87. PubMed
  8. Esteves C and Donadeu F. 2017. Cytometry A. 10.1002/cyto.a.23243. PubMed
  9. Ayhan S, et al. 2021. Journal of Cell Science. 134(6):. PubMed
  10. Liu Z, et al. 2021. Cells. 10:. PubMed
  11. Tanigawa K, et al. 2019. J Cell Physiol. 234:17280. PubMed
  12. Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed
  13. Ding S, et al. 2018. Sci Rep. 8:10808. PubMed
  14. Rogers MA, et al. 2022. Front Cardiovasc Med. 9:778919. PubMed
  15. Le AH, et al. 2021. Journal of Cell Biology. 220(9):. PubMed
RRID
AB_493026 (BioLegend Cat. No. 303015) AB_493025 (BioLegend Cat. No. 303016)

Antigen Details

Structure
Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α1-α6), also associates with CD51 (αV), and α7- α9, 130 kD
Distribution

Lymphocytes, monocytes, granulocytes (low), platelets, mast cells, fibroblasts, endothelial cells

Function
Cell-cell and cell-matrix interactions
Ligand/Receptor
VCAM-1, MAdCAM-1, ECM
Cell Type
Embryonic Stem Cells, Endothelial cells, Fibroblasts, Granulocytes, Lymphocytes, Mast cells, Mesenchymal Stem Cells, Monocytes, Platelets, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hemler M. 1990. Annu. Rev. Immunol. 8:365.
2. Hynes R. 1992. Cell 69:11.

Gene ID
3688 View all products for this Gene ID
UniProt
View information about CD29 on UniProt.org

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