Ultra-LEAF™ Purified anti-human CD18 Antibod
- 产品名称：
- Ultra-LEAF™ Purified anti-human CD18 Antibod
- 产品类别：
- 抗体
- 产品编号：
- 302115
- 产品应用：
- 302115
- Verified Reactivity
- Human
- Reported Reactivity
- Pig
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood.? It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: inhibition of cell adhesion and migration3,4. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) (Cat. No. 302116)?is recommended for functional assays.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Kishimoto T, et al. 1997. Leucocyte Typing VI. Garland Press. London.
- Van Epps DE, et al. 1989. J. Immunol. 143:3207. (Block)
- Meerschaert J, et al. 1994. J. Immunol. 152:1915. (Block)
- Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
- Sommaggio R, et al. 2012. J. Immunol. 188:2075. PubMed
- Valenzuela NM, et al. 2013. J. Immunol. 190:6635. PubMed
- Product Citations
-
- Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
- Cremasco F, et al. 2021. PLoS One. 16:e0241091. PubMed
- RRID
- AB_2814126 (BioLegend Cat. No. 302115) AB_2561483 (BioLegend Cat. No. 302116)
- Structure
- Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c, 90-95 kD
- Distribution
-
Leukocytes
- Function
- Adhesion and signaling in hematopoietic system
- Ligand/Receptor
- ICAMs
- Cell Type
- Leukocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Anderson D, et al. 1987. Annu. Rev. Med. 38:175.
2. Springer T. 1994. Cell 76:301.
- Gene ID
- 3689 View all products for this Gene ID
- UniProt
- View information about CD18 on UniProt.org
