Alexa Fluor® 700 anti-human CD18 Antibody
- 产品名称：
- Alexa Fluor® 700 anti-human CD18 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 302123
- 产品应用：
- 302123
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2226 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Pig
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: inhibition of cell adhesion and migration3,4. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) (Cat. No. 302116)?is recommended for functional assays.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Kishimoto T, et al. 1997. Leucocyte Typing VI. Garland Press. London.
- Van Epps DE, et al. 1989. J. Immunol. 143:3207. (Block)
- Meerschaert J, et al. 1994. J. Immunol. 152:1915. (Block)
- Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
- Sommaggio R, et al. 2012. J. Immunol. 188:2075. PubMed
- Valenzuela NM, et al. 2013. J. Immunol. 190:6635. PubMed
- Product Citations
-
- Reif T, et al. 2021. Cell Reports Medicine. 2(6):100317. PubMed
- RRID
- AB_2750394 (BioLegend Cat. No. 302123) AB_2750395 (BioLegend Cat. No. 302124)
- Structure
- Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c, 90-95 kD
- Distribution
-
Leukocytes
- Function
- Adhesion and signaling in hematopoietic system
- Ligand/Receptor
- ICAMs
- Cell Type
- Leukocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Anderson D, et al. 1987. Annu. Rev. Med. 38:175.
2. Springer T. 1994. Cell 76:301.
- Gene ID
- 3689 View all products for this Gene ID
- UniProt
- View information about CD18 on UniProt.org
