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首页 > 抗体蛋白 > 抗体
APC anti-mouse/human IL-5 Antibody
产品名称：
APC anti-mouse/human IL-5 Antibody
产品类别：
抗体
产品编号：
504305
产品应用：
504305
[价格]
规格 价格 库存
25µg ￥ 1428 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Guinea Pig
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partially-purified T cell clone supernatant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA1,3,4 or ELISPOT2,6 Capture: The purified TRFK5 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated TRFK4 antibody (Cat. No. 504402) as the detection antibody for mouse IL-5, or used in conjunction with biotinylated JES1-5A10 antibody (Cat. No. 501002) as the detection antibody for human IL-5. The Ultra-LEAF? purified antibody is suggested for ELISPOT capture.

Flow Cytometry: The fluorochrome-labeled TRFK51 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-5-producing cells within mixed cell populations.

Neutralization1,9: The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human IL-5 bioactivity (Cat. Nos. 504313-504318).

Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections7, and immunocytochemistry.

Note: For testing human IL-5 in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 430401 and 430404) are specially developed and recommended. For testing mouse IL-5 in serum, plasma or supernatant, BioLegend's ELISA MAX? Sets (Cat. Nos. 431201 and 431206) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
  2. Klinman, D., et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19.
  3. Swain, S., et al. 1990. J. Immunol. 145:3796.
  4. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
  5. Assenmacher, M., et al. 1994. Eur. J. Immunol. 24:1097.
  6. Karulin, A., et al. 2000. J. Immunol. 164:1862.
  7. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  8. Fan, W. Y., et al. 2001. Exp. Biol. Med. 226:1045.
  9. Sitkauskiene, B., et al. 2005. Respir Res. 6:33.
Product Citations
  1. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  2. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  3. Wei H, et al. 2021. Malar J. 20:89. PubMed
  4. Baldwin SL, et al. 2021. PLoS One. 16:e0247990. PubMed
  5. Shah RR, et al. 2019. Sci Rep. 9:11520. PubMed
  6. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  7. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  8. Boothby IC, et al. 2021. Nature. 599:667. PubMed
  9. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  10. Emg?rd J, et al. 2018. Immunity. 143:419. PubMed
  11. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  12. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  13. Nakajima T, et al. 2021. J Exp Med. 218:. PubMed
  14. Gu C, et al. 2021. Front Immunol. 12:678036. PubMed
  15. Schütze N, et al. 2016. J Immunol. 197: 2653 - 2664. PubMed
  16. DiPiazza AT, et al. 2021. Immunity. 54(8):1869-1882.e6. PubMed
  17. Corbett KS, et al. 2020. Nature. 586:567. PubMed
  18. Peng L, et al. 2022. Cell Rep Med. 3:100634. PubMed
  19. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  20. Colliou N, et al. 2015. Toxins. 7: 3805 - 3817. PubMed
  21. Chen Z, et al. 2016. Nat Commun. 7:11596. PubMed
  22. Williams J, et al. 2013. Am J Physiol Lung Cell Mol Physiol. 305:693. PubMed
  23. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  24. Singh A, et al. 2020. Cell Rep. 32:108153. PubMed
  25. Sun X, et al. 2017. Microbes Infect. 10.1016/j.micinf.2017.08.007. PubMed
  26. Baja?a S, et al. 2022. iScience. 25:103732. PubMed
  27. Schulze B, et al. 2016. Pathog Dis. 74: ftw020. PubMed
  28. Yu KK, et al. 2021. JCI Insight. 6:. PubMed
  29. Walker JA, et al. 2020. Immunity. 51(1):104-118. PubMed
  30. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
RRID
AB_315329 (BioLegend Cat. No. 504305) AB_315330 (BioLegend Cat. No. 504306)

Antigen Details

Structure
Cytokine; dimer; 40-50 kD (Mammalian)
Bioactivity
Growth/differentiation of B cells, eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Cell Sources
Mast cells, T cells, eosinophils
Cell Targets
Eosinophils, B cells
Receptors
Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107.
3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu, K. 1991. Microbiol. Immunol. 35:593.

Gene ID
16191 View all products for this Gene ID 3567 View all products for this Gene ID
UniProt
View information about IL-5 on UniProt.org

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