Biotin anti-mouse IL-5 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1414	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Partially-purified T cell clone supernatant

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-5 recombinant protein ranging from 250 to 1 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 ?g/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA^1-4 or ELISPOT^5-7 Detection: The biotinylated TRFK4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified TRFK5 antibody (Cat. No. 504302/504308) as the capture antibody.
Additional reported applications (for the relevant formats) include: neutralization¹ of mouse IL-5 bioactivity. The LEAF? purified TRFK5 antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of mouse and human IL-5 bioactivity (Cat. No. 504308).
Note: For testing mouse IL-5 in serum, plasma or supernatant, BioLegend's ELISA Max? Sets (Cat. No. 431201 to 431206) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

Schumacher, J., et al. 1988. J. Immunol. 141:1576.
Abrams, J., et al. 1992. Immunol. Rev. 127:5.
Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
Sander, B., et al. 1993. J. Immunol. Meth. 166:201.
Taguchi, T., et al. 1990. J. Immunol. Meth. 128:65.
Taguchi, T., et al. 1990. J. Immunol. 145:68.
Klinman, D., et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19.

Product Citations

Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed

RRID

AB_2296156 (BioLegend Cat. No. 504401) AB_2127162 (BioLegend Cat. No. 504402)

Antigen Details

Structure: Cytokine; dimer; 40-50 kD (Mammalian)
Bioactivity: Growth/differentiation of B cells, eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Cell Sources: Mast cells, T cells, eosinophils
Cell Targets: Eosinophils, B cells
Receptors: Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107.
3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu, K. 1991. Microbiol. Immunol. 35:593.
Gene ID: 16191 View all products for this Gene ID
UniProt: View information about IL-5 on UniProt.org

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