Ultra-LEAF™ Purified anti-human CD354 (TREM-
- 产品名称：
- Ultra-LEAF™ Purified anti-human CD354 (TREM-
- 产品类别：
- 抗体
- 产品编号：
- 314913
- 产品应用：
- 314913
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human TREM-1
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Activ, WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Detection: The biotinylated TREM-26 antibody is useful as a detection antibody for a TREM-1 specific sandwich ELISA, when used in conjunction with the TREM-37 antibody (Cat. No. 316102) as the capture antibody. The TREM-26 antibody is useful for flow cytometry and activation of monocytes and granulocytes. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 314913 & 314914).
- Application References
(PubMed link indicates BioLegend citation) -
- Li J, et al. 2011. Dev. Comp. Immunol. epub.
- RRID
- AB_2814167 (BioLegend Cat. No. 314913) AB_2814168 (BioLegend Cat. No. 314914)
- Structure
- Type I membrane protein, 30 kD, contains immunoglobulin-like V-type domain. Alternatively spliced variant may be secreted.
- Distribution
-
Highly expressed in adult liver, lung, and spleen, lower expression in lymph node, placenta, spinal cord, and heart. High expression in peripheral blood myeloid cells.
- Function
- Stimulates neutrophil and monocyte-mediated inflammatory responses through the triggering and release of pro-inflammatory cytokines and chemokines and the upregulation of surface activation markers. Amplifies inflammatory responses to fungal and bacterial
- Interaction
- DAP12
- Modification
- Glycosylated
- Cell Type
- Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Cytokines/Chemokines
- Antigen References
-
1. Bouchon A, et al. 2001. Nature 410:1103.
2. Bouchon A, et al. 2000. J. Immunol. 164:4991.
3. Gingras MC, et al. 2002. Mol. Immunol. 38:817.
- Regulation
- Upregulated by bacteria, fungi, and LPS
- Gene ID
- 54210 View all products for this Gene ID
- UniProt
- View information about CD354 on UniProt.org
