TotalSeq™-C0858 anti-human CD46 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
10µg	￥ 5166	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

2102Ep human embryonal carcinoma cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA

Preparation

The antibody was purified by chromatography and conjugated with TotalSeq?-C oligomer under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling?Solution.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume. Refer to the corresponding TotalSeq? protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence staining¹, immunoprecipitation¹, and blocking of measles virus infection². The epitope of TRA-2-10 is in the SCR-1 domain of CD46.

Additional Product Notes

TotalSeq? reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g.?10x Genomics Chromium System and Reagents) and sequencer (e.g Illumina analyzers) are required. Please contact?technical support?for more information, or visit?biolegend.com/totalseq.

The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.

Application References (PubMed link indicates BioLegend citation)

Cardone J, et al. 2010. Nat. Immunol. 11:862. (IF, IP)
Blau DM, Compans RW. 1995. Virology. 210:91. (Block)

RRID

AB_2927946 (BioLegend Cat. No. 352417)

Antigen Details

Structure: Transmembrane receptor, contains 4 SUSHI domains, multiple isoforms exist ranging in molecular weight from 45-75 kD. Larger isoforms from 65-75 kD are not expressed on peripheral blood cells.
Distribution: Widely expressed on leukocytes, platelets, epithelial cells, and fibroblasts.
Function: Binds complement components C3B and C4B with cofactor activity for the I-dependent cleavage of these proteins. May also play a role in T cell regulation.
Interaction: Human 55-73 kD polypeptide encoded by human chromosome 1.
Cell Targets: Moesin, c-Src, complement component 3, complement component 4B, tetraspanin 4, c-Yes, Integrin β1
Receptors: C3B, C4B, Measles virus, Human herpes virus-6
Biology Area: Cell Biology, Complement, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Cardone J, et al. 2010. Nat. Immunol. 11:862.
2. Dorig RE, et al. 1993. Cell 75:295.
3. Kemper C, et al. 2003. Nature 421:388.
4. Frecha C, et al. 2011. J. Virol. 85:5975.
5. Adams EM, et al. 1991. J. Immunol. 147:3005.
Gene ID: 4179 View all products for this Gene ID
UniProt: View information about CD46 on UniProt.org

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