Purified anti-human CD46 Antibody
- 产品名称：
- Purified anti-human CD46 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 352403
- 产品应用：
- 352403
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- 2102Ep human embryonal carcinoma cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICC, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence staining1, immunoprecipitation1, and blocking of measles virus infection2. The epitope of TRA-2-10 is in the SCR-1 domain of CD46.
- Application References
(PubMed link indicates BioLegend citation) -
- Cardone J, et al. 2010. Nat. Immunol. 11:862. (IF, IP)
- Blau DM, Compans RW. 1995. Virology. 210:91. (Block)
- Product Citations
-
- Kuppan JP, et al. 2021. Elife. 10:. PubMed
- RRID
- AB_10897448 (BioLegend Cat. No. 352403) AB_10900243 (BioLegend Cat. No. 352404)
- Structure
- Transmembrane receptor, contains 4 SUSHI domains, multiple isoforms exist ranging in molecular weight from 45-75 kD. Larger isoforms from 65-75 kD are not expressed on peripheral blood cells.
- Distribution
-
Widely expressed on leukocytes, platelets, epithelial cells, and fibroblasts.
- Function
- Binds complement components C3B and C4B with cofactor activity for the I-dependent cleavage of these proteins. May also play a role in T cell regulation.
- Interaction
- Human 55-73 kD polypeptide encoded by human chromosome 1.
- Cell Targets
- Moesin, c-Src, complement component 3, complement component 4B, tetraspanin 4, c-Yes, Integrin β1
- Receptors
- C3B, C4B, Measles virus, Human herpes virus-6
- Biology Area
- Cell Biology, Complement, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Cardone J, et al. 2010. Nat. Immunol. 11:862.
2. Dorig RE, et al. 1993. Cell 75:295.
3. Kemper C, et al. 2003. Nature 421:388.
4. Frecha C, et al. 2011. J. Virol. 85:5975.
5. Adams EM, et al. 1991. J. Immunol. 147:3005.
- Gene ID
- 4179 View all products for this Gene ID
- UniProt
- View information about CD46 on UniProt.org
