PE anti-mouse CD120b (TNF R Type II/p75) Antibody
- 产品名称：
- PE anti-mouse CD120b (TNF R Type II/p75) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 113405
- 产品应用：
- 113405
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- E. coli -expressed mouse Type II TNFR
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1. The TR75-89 antibody does not block TNF-α binding to receptor. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113406) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 113404) or biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)).
- Application References
(PubMed link indicates BioLegend citation) -
- Sheehan KC, et al. 1995. J. Exp. Med. 181:607. (IP)
- Schlaepfer DD, et al. 2007. J. Biol. Chem. 282:17450. (FC)
- Bell JH, et al. 2007. J. Leukoc. Biol. 82:173. (FC)
- Product Citations
-
- Ohta S, et al. 2011. PLoS One. 6:e16182. PubMed
- Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
- Baram T, et al. 2021. Cells. 10:. PubMed
- Lindborg JA et al. 2017. The Journal of Neuroscience. 37(43):10258-10277 . PubMed
- Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
- Jiang Y, et al. 2019. J Immunother Cancer. 7:28. PubMed
- Akagi T, et al. 2022. Front Immunol. 13:926175. PubMed
- Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
- Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
- Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
- Akhtar MN, et al. 2020. PLoS Pathog. 16:e1009136. PubMed
- RRID
- AB_2206942 (BioLegend Cat. No. 113405) AB_2206941 (BioLegend Cat. No. 113406)
- Structure
- TNFR superfamily, 75 kD
- Distribution
- Variety of cell types at low levels
- Function
- Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
- Ligand/Receptor
- TNF-α, LT-α (TNF-β)
- Cell Type
- Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Aggarwal BB, et al. 1985. Nature 318 665.
2. Chan FKM, et al. 2000. Science 288:2351.
3. Loetscher H, et al. 1990. Cell 61:351.
4. Rothe J, et al. 1993. Nature 364:798.
- Gene ID
- 21938 View all products for this Gene ID
- UniProt
- View information about CD120b on UniProt.org
