上海细胞库
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
| 规格 | 价格 | 库存 |
|---|---|---|
| 50µg | ￥ 1484 | 1 |
ELISA, FC - Quality tested
IP - Reported in the literature, not verified in house
Each lot of this antibody is quality control tested by ELISA assay and/or immunofluorescent staining with flow cytometric analysis For ELISA detection applications, a concentration range of 0.125-0.5 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of TNF R Type II/p75 recombinant protein ranging from 250 to 4 pg/ml are recommended for each ELISA plate. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Additional reported applications (for the relevant formats) include: immunoprecipitation1. The TR75-89 antibody does not block TNF-α binding to receptor. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113406) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 113404) or biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)).
1. Aggarwal BB, et al. 1985. Nature 318 665.
2. Chan FKM, et al. 2000. Science 288:2351.
3. Loetscher H, et al. 1990. Cell 61:351.
4. Rothe J, et al. 1993. Nature 364:798.
