Ultra-LEAF™ Purified anti-mouse/rat TNF-&alp-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1540	1

Product Details

Verified Reactivity

Mouse, Rat

Reported Reactivity

Rabbit

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

E. coli-expressed, recombinant mouse TNF-α

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
ELISPOT Capture, Neut, ICFC, IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2 - 6 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse TNF-α recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture¹: The Ultra-LEAF? Purified TN3-19.12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated Poly5160 antibody (Cat. No. 516003) as the detecting antibody for detecting mouse/rat TNF-a. The Ultra-LEAF? purified antibody is suggested for ELISPOT capture.

Clone TN3-19.12 has been shown to neutralize murine TNF-a and cross reacts wtih mouse TFN-?¹. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Aside free, 0.2 ?m filtered) is recommended for neutralization (Cat. Nos. 506109 - 506114).

Application References (PubMed link indicates BioLegend citation)

Sheehan, K., et al. 1989. J. Immunol. 142:3884. (Neut)
Merrick, B.A., et al. 1992. Appl. Theor. Electrophor 2:177.
Takahashi, S., et al. 1995. Brain Res 690:241.
Rabinovich, R., et al. 1993. J. Pharmacol. Exp. Ther. 267:1550.
Molloy, R., et al. 1993. J. Immunol. 151:2142.
Finkelman, F., et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28.
Simkins, HM., et al. 2011. J. Leukoc Biol. 89:753. PubMed.
Faas MM, et al. 2003. J. Reprod. Immunol. 1:39-52.

RRID

AB_2820092 (BioLegend Cat. No. 506109) AB_2820093 (BioLegend Cat. No. 506110) AB_2832810 (BioLegend Cat. No. 506111) AB_2832811 (BioLegend Cat. No. 506112) AB_2832812 (BioLegend Cat. No. 506113) AB_2832813 (BioLegend Cat. No. 506114)

Antigen Details

Structure: TNF superfamily; dimer/trimer; 18-150 kD (Mammalian)
Bioactivity: Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; endothelial cell alterations; chemoattractant
Cell Sources: Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Targets: Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
Receptors: TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Cell Type: Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler, B., et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler, B., et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey, K., et al. 1993. Crit. Care Med. 21:S415.
Regulation: Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists,
Gene ID: 21926 View all products for this Gene ID 24835 View all products for this Gene ID
UniProt: View information about TNF-alpha on UniProt.org

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