PE anti-mouse / rat TNF-α Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3528	1

Product Details

Verified Reactivity

Mouse, Rat

Reported Reactivity

Rabbit

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

E. coli-expressed, recombinant mouse TNF-α

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It isrecommended that the reagent be titrated for optimalperformance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

ELISA or ELISPOT Capture¹: The Ultra-LEAF? Purified TN3-19.12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated Poly5160 antibody (Cat. No. 516003) as the detecting antibody for detecting mouse/rat TNF-a. The Ultra-LEAF? purified antibody is suggested for ELISPOT capture.

Clone TN3-19.12 has been shown to neutralize murine TNF-a and cross reacts wtih mouse TFN-?¹. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Aside free, 0.2 ?m filtered) is recommended for neutralization (Cat. Nos. 506109 - 506114).

Application References (PubMed link indicates BioLegend citation)

Sheehan, K., et al. 1989. J. Immunol. 142:3884. (Neut)
Merrick, B.A., et al. 1992. Appl. Theor. Electrophor 2:177.
Takahashi, S., et al. 1995. Brain Res 690:241.
Rabinovich, R., et al. 1993. J. Pharmacol. Exp. Ther. 267:1550.
Molloy, R., et al. 1993. J. Immunol. 151:2142.
Finkelman, F., et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28.
Simkins, HM., et al. 2011. J. Leukoc Biol. 89:753. PubMed.
Faas MM, et al. 2003. J. Reprod. Immunol. 1:39-52.

RRID

AB_315418 (BioLegend Cat. No. 506104)

Antigen Details

Structure: TNF superfamily; dimer/trimer; 18-150 kD (Mammalian)
Bioactivity: Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; endothelial cell alterations; chemoattractant
Cell Sources: Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Targets: Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
Receptors: TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Cell Type: Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler, B., et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler, B., et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey, K., et al. 1993. Crit. Care Med. 21:S415.
Regulation: Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists,
Gene ID: 21926 View all products for this Gene ID 24835 View all products for this Gene ID
UniProt: View information about TNF-alpha on UniProt.org

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