PE anti-mouse CD122 (IL-2Rβ) Antibody
- 产品名称：
- PE anti-mouse CD122 (IL-2Rβ) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 123209
- 产品应用：
- 123209
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Rat T cell line expressing mouse CD122 ( IL-2Rβ)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking of IL-2 binding1, and NK cell depletion2 in vivo. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for blocking of IL-2 binding in vivo and in vitro (Cat. No. 123204).
- Application References
(PubMed link indicates BioLegend citation) -
- Tanaka T, et al. 1991. J. Immunol. 147:2222.
- Tanaka T, et al. 1993. J. Exp. Med. 178:1103.
- Tanaka T, et al. 1992. Int. Immunol. 4:487.
- Product Citations
-
- Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
- Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
- Reyes RM, et al. 2021. Oncoimmunology. 10:2006529. PubMed
- Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
- Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
- De Simone G, et al. 2021. Immunity. :. PubMed
- Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
- Yue X, et al. 2021. EMBO Rep. 22:e52716. PubMed
- Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
- Hu Z, et al. 2013. J Immunol. 191:312. PubMed
- Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
- Long L, et al. 2021. Nature. 600:308. PubMed
- Wu C, et al. 2018. Scand J Immunol. 87:e12661. PubMed
- RRID
- AB_940615 (BioLegend Cat. No. 123209) AB_940617 (BioLegend Cat. No. 123210)
- Structure
- Ig superfamily, forms high affinity IL-2 receptor with CD25 and CD132 chains or intermediate affinity receptor with CD132 alone, 70-75 kD
- Distribution
- T and B cells, NK cells, monocytes, macrophages
- Function
- Critical component of IL-2 and IL-15 signaling
- Ligand/Receptor
- IL-2, IL-15
- Cell Type
- B cells, Macrophages, Monocytes, NK cells, T cells, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245.
3. Suzuki H, et al. 1995. Science 268:1472.
4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109.
- Gene ID
- 16185 View all products for this Gene ID
- UniProt
- View information about CD122 on UniProt.org
