Biotin anti-mouse CD122 (IL-2Rβ) Antibody
- 产品名称：
- Biotin anti-mouse CD122 (IL-2Rβ) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 123205
- 产品应用：
- 123205
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Rat T cell line expressing mouse CD122 ( IL-2Rβ)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking of IL-2 binding1, and NK cell depletion2 in vivo. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for blocking of IL-2 binding in vivo and in vitro (Cat. No. 123204).
- Application References
(PubMed link indicates BioLegend citation) -
- Tanaka T, et al. 1991. J. Immunol. 147:2222.
- Tanaka T, et al. 1993. J. Exp. Med. 178:1103.
- Tanaka T, et al. 1992. Int. Immunol. 4:487.
- Product Citations
-
- Schuster M, et al. 2017. J Immunol. 199:920. PubMed
- Pobezinskaya EL et al. 2019. Front Immunol. 1.079861111 . PubMed
- Maine C, et al. 2012. J Immunol. 188:5267. PubMed
- Wells AC et al. 2017. eLife. 6 pii: e26398. PubMed
- Schuster M, et al. 2019. Front Immunol. 10:1583. PubMed
- RRID
- AB_940607 (BioLegend Cat. No. 123205) AB_940609 (BioLegend Cat. No. 123206)
- Structure
- Ig superfamily, forms high affinity IL-2 receptor with CD25 and CD132 chains or intermediate affinity receptor with CD132 alone, 70-75 kD
- Distribution
-
T and B cells, NK cells, monocytes, macrophages
- Function
- Critical component of IL-2 and IL-15 signaling
- Ligand/Receptor
- IL-2, IL-15
- Cell Type
- B cells, Macrophages, Monocytes, NK cells, T cells, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245.
3. Suzuki H, et al. 1995. Science 268:1472.
4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109.
- Gene ID
- 16185 View all products for this Gene ID
- UniProt
- View information about CD122 on UniProt.org
