Alexa Fluor® 488 anti-human CD282 (TLR2) Antib-上海雅吉生物科技有限公司

规格	价格	库存
100tests	￥ 4102	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human TLR2-transfected CHO cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Application Notes

The TL2.1 antibody is useful for blocking studies. It has been reported to block TLR2 agonist-induced cellular activation. Additional reported applications (for the relevant formats) include: inhibition of PGP activity and blocking of cytokine production^1,3,7, immunoprecipitation¹, immunohistochemistry of 4% paraformaldehyde-fixed frozen sections² and immunohistochemistry of HOPE-fixed (HEPES-glutamic acid buffer-mediated organic solvent protection effect) paraffin-embedded sections⁴ , and Western blotting². For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 309716) (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered).

Application References (PubMed link indicates BioLegend citation)

Flo T, et al. 2000. J. Immunol. 164:2064.
Faure E, et al. 2001. J. Immunol. 166:2018.
Sugawara S, et al. 2001. Infect. Immun. 69:4951.
Droemann D, et al. 2003. Histochem. Cell Biol. 119:103.
Chavakis E, et al. 2007. Circ. Res. 100:204.PubMed
Fiala M, et al. 2007. Proc. Natl. Acad. Sci. USA 10.1073/P. Natl. Acad. Sci. USA.0701267104.
Goo SY, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M701876200.PubMed
Weiss DJ,et al. 2008.J. Leukoc. Biol. 83:48. PubMed
Harris, KM., et al. 2011. J. Leukoc Biol. 90:727. PubMed.
Elass-Rocahrd E, et al. 2012. J Biol Chem. 287:34432. PubMed.

Product Citations

Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed

RRID

AB_2204582 (BioLegend Cat. No. 309712)

Antigen Details

Structure: IL-1R superfamily, TLR family, type 1 transmembrane protein, 90 kD
Distribution: Monocytes, granulocytes, endothelial and epithelial cells
Function: Activates innate host defense against bacterial pathogens
Ligand/Receptor: Microbial lipoproteins, peptidoglycans, lipoteichoic acid
Cell Type: Endothelial cells, Epithelial cells, Granulocytes, Monocytes, Neutrophils, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Toll Like Receptors
Antigen References: 1. Lien E, et al. 1999. J. Biol. Chem. 274:33419.
2. Lien E, et al. 2001. J. Biol. Chem. 276:1873.
3. Sabroe I, et al. 2002. J. Immunol. 168:4701
Gene ID: 7097 View all products for this Gene ID
UniProt: View information about CD282 on UniProt.org

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