Ultra-LEAF™ Purified anti-mouse 4-1BB Ligand-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1540	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

BALB/c mouse B lymphoma line 2PK-3

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The TKS-1 antibody inhibits binding of soluble 4-1BB to 4-1BBL in vitro. Additional reported applications (for the relevant formats) include: in vitro blocking the binding of 4-1BB to 4-1BBL¹. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107106) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107104) followed by SAv-PE (Cat. No. 405204)). The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 107111-107116).

Application References (PubMed link indicates BioLegend citation)

Akiba H, et al. 2000. J. Exp. Med. 191:375. (Block)
Futagawa T, et al. 2002. Int. Immunol. 14:275.
Nishimoto H, et al. 2005. Blood 106:4241. (FC) PubMed
De Keersmaecker B, et al. 2011. J Leukoc Biol. 89:989. PubMed.
Zhao Y, et al. 2012. Immuno Lett. 141:220. PubMed.

RRID

AB_2832355 (BioLegend Cat. No. 107111) AB_2832356 (BioLegend Cat. No. 107112) AB_2832357 (BioLegend Cat. No. 107113) AB_2832358 (BioLegend Cat. No. 107114) AB_2832359 (BioLegend Cat. No. 107115) AB_2894664 (BioLegend Cat. No. 107116)

Antigen Details

Structure: NGF/TNF superfamily, 97 kD
Distribution: Activated B cells and T cells, macrophages, and dendritic cells
Function: T cell costimulation
Ligand/Receptor: 4-1BB (CDw137)
Cell Type: B cells, Dendritic cells, Macrophages, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Pollok KE, et al. 1995. Eur. J. Immunol. 25:488.
2. DeBenedette MA, et al. 1997. J. Immunol. 158:551.
3. Pollok KE, et al. 1993. J. Immunol. 150:771.
4. Pollok KE, et al. 1994. Eur. J. Immunol. 24:367.
5. Goodwin RG, et al. 1993. Eur. J. Immunol. 23:2631.
Gene ID: 21942 View all products for this Gene ID
UniProt: View information about CD137L on UniProt.org

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