Ultra-LEAF™ Purified anti-mouse FR4 (Folate
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse FR4 (Folate
- 产品类别：
- 抗体
- 产品编号：
- 125111
- 产品应用：
- 125111
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Cyt, Depletion - Reported in the Literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
1. Yamaguchi T et al.?1007: Immunity. 27(1)145
2. Inoue N, et al. 2015. Nat Commun. 6: 8858. PubMed
3. Kato K, et al. 2016. Nat Commun. 7:12198. PubMed
- RRID
- AB_2890682 (BioLegend Cat. No. 125111) AB_2890682 (BioLegend Cat. No. 125112)
- Structure
- 28 kd protein consisting of 244 amino acids. Heavily glycosylated
- Distribution
-
Expressed on mouse thymocytes and spleen cells. Highly expressed on natural regulatory (Treg) cells
- Function
- Mediate calcium-independent homophlic cell-cell adhesion
- Ligand/Receptor
- Folic acid and its derivative
- Cell Type
- Thymocytes, Tregs
- Biology Area
- Immunology, Innate Immunity
- Antigen References
-
1. Yamaguchi T, et al. 2007. Immunity. 27:145
2. Spiegelstein O,?et al. 2000.?Gene. 258:117
- Gene ID
- 64931 View all products for this Gene ID
- UniProt
- View information about FR4 on UniProt.org
