Ultra-LEAF™ Purified anti-mouse TER-119/Eryt-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1540	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Day-14 fetal liver cells from a C57BL/6 mouse

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC-F, IHC-P, IP, WB, CMCD - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting¹, complement-mediated cytotoxicity³, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References (PubMed link indicates BioLegend citation)

Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
Vannucchi AM, et al. 2000. Blood 95:2559.
Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
Grisendi S, et al. 2005. Nature 437:147. (FC)
Bourdeau A, et al. 2007. Blood 109:4220.
Chappaz S, et al. 2007. Blood 110:3862. (FC)
Heuser M, et al. 2007. Blood 110:1639. (FC)
Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed

Product Citations

Formaglio P, et al. 2021. Immunity. 54:2724. PubMed

RRID

AB_2832399 (BioLegend Cat. No. 116253) AB_2832400 (BioLegend Cat. No. 116254) AB_2832401 (BioLegend Cat. No. 116255) AB_2832402 (BioLegend Cat. No. 116256) AB_2832403 (BioLegend Cat. No. 116257) AB_2832404 (BioLegend Cat. No. 116258)

Antigen Details

Structure: Associated with glycophorin A, 52 kD
Distribution: Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E
Cell Type: Erythrocytes
Biology Area: Immunology
Antigen References: 1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.
Gene ID: 104231 View all products for this Gene ID
UniProt: View information about TER-119 on UniProt.org

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