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Purified anti-mouse TER-119/Erythroid Cells Antibo
产品名称:
Purified anti-mouse TER-119/Erythroid Cells Antibo
产品类别:
抗体
产品编号:
116201
产品应用:
116201
[价格]
规格 价格 库存
50µg ¥ 588 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IHC-P, IP, WB?- Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Shafer MER, et al. 2017. Stem Cell Reports. 1.040277778. PubMed
  2. Chamberlin T, et al. 2020. Cancer Research. 80(20):4465-4475. PubMed
  3. Patel MM, et al. 2018. J Am Heart Assoc. 7:e010690. PubMed
  4. Baganha F, et al. 2022. Int J Mol Sci. 23:. PubMed
  5. Yanagida K, et al. 2020. Dev Cell. 779:52. PubMed
  6. Chen L, et al. 2019. Nat Commun. 3.553472222. PubMed
  7. Nguyen N, et al. 2022. iScience. 25:103679. PubMed
  8. Motoyama S, et al. 2020. Cells. :9. PubMed
  9. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  10. Peng V, et al. 2020. J Biol Chem. 295:14866. PubMed
  11. Chen S, et al. 2018. Immunohorizons. 0.345138889. PubMed
  12. Blume J, et al. 2019. Eur J Immunol. 49:121. PubMed
  13. Heuser M, et al. 2007. Blood. 110:1639. PubMed
  14. Suzuki Y, et al. 2021. FEBS Open Bio. 11:2619. PubMed
  15. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  16. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  17. Chappaz S, et al. 2007. Blood. 110:3862. PubMed
  18. Brown IK, et al. 2021. PLoS Pathog. 17:e1009602. PubMed
  19. Niaudet C, et al. 2019. Angiogenesis. 1.257638889. PubMed
  20. Tahara N, et al. 2018. Dev Biol. 434:74. PubMed
  21. Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
  22. Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
  23. You X, et al. 2022. Front Genet. 12:790990. PubMed
  24. Duong CN, et al. 2020. Arterioscler Thromb Vasc Biol. 40:378. PubMed
  25. Malik N, et al. 2019. Sci Rep. 9:16917. PubMed
  26. Wang HC, et al. 2017. J Immunol. 198:3149. PubMed
  27. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  28. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  29. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
  30. Lin Z, et al. 2021. Stem Cells. 39:240. PubMed
  31. Goltsev Y et al. 2018. Cell. 174(4):968-981 . PubMed
  32. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  33. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  34. Heib T, et al. 2021. Cell Reports. 35(6):109102. PubMed
  35. Cheng P, et al. 2008. J Exp Med. 205:2235. PubMed
  36. Gui J, et al. 2007. Int Immunol. 1.625694444. PubMed
  37. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  38. Lin J, et al. 2017. Nat Commun. . 10.1038/s41467-017-01477-5. PubMed
  39. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  40. Gough S, et al. 2014. Cancer Discovery. 4:564. PubMed
RRID
AB_313702 (BioLegend Cat. No. 116201) AB_313703 (BioLegend Cat. No. 116202)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

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