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首页 > 抗体蛋白 > 抗体
PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cell
产品名称：
PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cell
产品类别：
抗体
产品编号：
116227
产品应用：
116227
[价格]
规格 价格 库存
25µg ￥ 1652 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 116253-116258).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  2. Krausgruber T, et al. 2020. Nature. 583:296. PubMed
  3. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
  4. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  5. De S, et al. 2018. Development. 145. PubMed
  6. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  7. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  8. Alcicek FC, et al. 2022. Front Physiol. 13:839323. PubMed
  9. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  10. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  11. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  12. Bai Y, et al. 2021. Front Cell Dev Biol. 9:730095. PubMed
  13. Kobayashi M, et al. 2020. Bio Protoc. 10:e3601. PubMed
  14. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  15. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  16. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  17. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
  18. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  19. Mesnieres M, et al. 2021. Cell Reports. 36(8):109618. PubMed
  20. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  21. B?hm AM et al. 2019. Dev Cell. 51(2):236-254 . PubMed
  22. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  23. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
  24. S?wen P et al. 2018. eLife. 7 pii: e41258. PubMed
  25. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  26. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
RRID
AB_893638 (BioLegend Cat. No. 116227) AB_893636 (BioLegend Cat. No. 116228)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

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