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PE/Cyanine7 anti-mouse TER-119/Erythroid Cells Ant
产品名称：
PE/Cyanine7 anti-mouse TER-119/Erythroid Cells Ant
产品类别：
抗体
产品编号：
116221
产品应用：
116221
[价格]
规格 价格 库存
25µg ￥ 1414 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 116253-116258).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  2. Sch?nberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  3. Maimets M, et al. 2016. Stem Cell Reports. 6:150-162. PubMed
  4. Sun C, et al. 2020. Cell Stem Cell. 26(5):707-721. PubMed
  5. Wang Y, et al. 2021. J Bone Oncol. 30:100386. PubMed
  6. Fu X, et al. 2015. J Biol Chem. 290: 26445 - 26456. PubMed
  7. Silva C, et al. 2019. Cell Physiol Biochem. 52:503. PubMed
  8. Al-Rifai R, et al. 2022. Nat Commun. 13:6592. PubMed
  9. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  10. Serrano Martinez P, et al. 2022. iScience. 25:105047. PubMed
  11. Huang P, et al. 2020. Blood. 135:2121. PubMed
  12. Jakob L, et al. 2021. Int J Mol Sci. 22:. PubMed
  13. Yoshida K, et al. 2022. Sci Rep. 12:17276. PubMed
  14. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  15. Wilmore JR et al. 2018. Cell host & microbe. 23(3):302-311 . PubMed
  16. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  17. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  18. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  19. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  20. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  21. van Gastel N, et al. 2020. Cell Metabolism. 32(3):391-403.e6. PubMed
  22. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  23. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  24. Sch?nberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  25. Xie X, et al. 2015. Sci Rep. 5: 18115. PubMed
  26. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  27. Dong H, et al. 2022. Nat Metab. 4:90. PubMed
  28. Feola M, et al. 2021. Commun Biol. 4:517. PubMed
  29. Orhon I, et al. 2021. Autophagy. :1. PubMed
RRID
AB_2137789 (BioLegend Cat. No. 116221) AB_2281408 (BioLegend Cat. No. 116222)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

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