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PE/Cyanine5 anti-mouse TER-119/Erythroid Cells Ant
产品名称：
PE/Cyanine5 anti-mouse TER-119/Erythroid Cells Ant
产品类别：
抗体
产品编号：
116209
产品应用：
116209
[价格]
规格 价格 库存
25µg ￥ 1162 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 116253-116258).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Nita A, et al. 2021. Cell Reports. 34(5):108688. PubMed
  2. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
  3. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  4. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  5. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  6. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  7. Chen I, et al. 2016. Sci Rep. 6:27195. PubMed
  8. Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
  9. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  10. Dawar S, et al. 2016. Cell Death Dis. 7:e2509. PubMed
  11. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  12. Yankova E, et al. 2021. Nature. 593:597. PubMed
  13. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  14. Nacson J, et al. 2020. Molecular Cell. 78(5):951-959. PubMed
  15. Liang G, et al. 2021. STAR Protoc. 2:100884. PubMed
  16. Dulken BW, et al. 2019. Nature. 571:205. PubMed
  17. Hohsfield LA, et al. 2020. J Neuroinflammation. 0.902083333. PubMed
  18. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
  19. Tzelepis K, et al. 2018. Nat Commun. 9:5378. PubMed
  20. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  21. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  22. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  23. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
  24. Koide S, et al. 2022. iScience. 25:103603. PubMed
  25. Heuser M, et al. 2009. Blood. 113:1432. PubMed
  26. S?wen P et al. 2018. eLife. 7 pii: e41258. PubMed
  27. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  28. Chavez JS, et al. 2022. Cells. 11:. PubMed
  29. Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed
  30. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  31. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
RRID
AB_313710 (BioLegend Cat. No. 116209) AB_313711 (BioLegend Cat. No. 116210)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

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