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首页 > 抗体蛋白 > 抗体
Pacific Blue™ anti-mouse TER-119/Erythroid C
产品名称：
Pacific Blue™ anti-mouse TER-119/Erythroid C
产品类别：
抗体
产品编号：
116231
产品应用：
116231
[价格]
规格 价格 库存
25µg ￥ 1848 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Zabala M, et al. 2020. Cell Stem Cell. 27(2):284-299.e8. PubMed
  2. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  3. Schneider C, et al. 2018. Cell. 174:271. PubMed
  4. Reinitz F, et al. 2022. Elife. 11:. PubMed
  5. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  6. Sperber HS, et al. 2020. FASEB J. 14615:34. PubMed
  7. Karaman S, et al. 2022. J Exp Med. 219:. PubMed
  8. Greenwald AC, et al. 2019. J Exp Med. 216:215. PubMed
  9. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
  10. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
  11. Diéguez–Hurtado R, et al. 2019. Nat Commun. 10:2817. PubMed
  12. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  13. Burr ML, et al. 2019. Cancer Cell. 36:385. PubMed
  14. Behling K, et al. 2016. J Nucl Med. 57: 1771 - 1777. PubMed
  15. Sato K, et al. 2017. J Immunol. 10.4049/jimmunol.1602005. PubMed
  16. Kato K, et al. 2018. Nat Commun. 48:120. PubMed
  17. Cuadrado M, et al. 2022. Sci Rep. 12:10489. PubMed
  18. Borrelli MR, et al. 2020. Stem Cells Transl Med. 1.347916667. PubMed
  19. Hemanthakumar KA, et al. 2021. eLife. 10:00. PubMed
  20. Eilken H, et al. 2017. Nat Commun. . 10.1038/s41467-017-01738-3. PubMed
  21. Shue YT, et al. 2022. Nat Commun. 13:2690. PubMed
RRID
AB_2149212 (BioLegend Cat. No. 116231) AB_2251160 (BioLegend Cat. No. 116232)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

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