Brilliant Violet 650™ anti-mouse TER-119/Ery-上海雅吉生物科技有限公司

规格	价格	库存
125µL	￥ 3080	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Day-14 fetal liver cells from a C57BL/6 mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650? excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting¹, complement-mediated cytotoxicity³, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References (PubMed link indicates BioLegend citation)

Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
Vannucchi AM, et al. 2000. Blood 95:2559.
Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
Grisendi S, et al. 2005. Nature 437:147. (FC)
Bourdeau A, et al. 2007. Blood 109:4220.
Chappaz S, et al. 2007. Blood 110:3862. (FC)
Heuser M, et al. 2007. Blood 110:1639. (FC)
Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed

Product Citations

Sch?nberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed

RRID

AB_11204244 (BioLegend Cat. No. 116235)

Antigen Details

Structure: Associated with glycophorin A, 52 kD
Distribution: Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E
Cell Type: Erythrocytes
Biology Area: Immunology
Antigen References: 1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.
Gene ID: 104231 View all products for this Gene ID
UniProt: View information about TER-119 on UniProt.org

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