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Brilliant Violet 421™ anti-mouse TER-119/Ery
产品名称：
Brilliant Violet 421™ anti-mouse TER-119/Ery
产品类别：
抗体
产品编号：
116233
产品应用：
116233
[价格]
规格 价格 库存
125µL ￥ 2702 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
?g sizes: 0.2 mg/mL
?L sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. For flow cytometric staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Cui Z, et al. 2022. Bone Res. 10:58. PubMed
  2. Romera–Hernández M, et al. 2020. Cell Reports. 30(1):37-45.e3.. PubMed
  3. Khoramian Tusi B, et al. 2018. Exp Hematol. 60:21. PubMed
  4. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  5. Chuang CH, et al. 2020. Cancer Research. 81(3):567-579. PubMed
  6. Yang YS, et al. 2020. Mol Ther Methods Clin Dev. 17:922. PubMed
  7. Ma Z, et al. 2022. Methods Mol Biol. 2471:49. PubMed
  8. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  9. Ombrato L, et al. 2021. Nat Protoc. 16:872. PubMed
  10. Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
  11. Jolly A, et al. 2022. Cell Rep Methods. 2:100315. PubMed
  12. Nagatake T, et al. 2021. Int Immunol. 33:171. PubMed
  13. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
  14. Hu B, et al. 2020. J Clin Invest. 130:3483. PubMed
  15. Peltzer N, et al. 2018. Nature. 557:112. PubMed
  16. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
RRID
AB_10933426 (BioLegend Cat. No. 116233) AB_2562917 (BioLegend Cat. No. 116234)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

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