Alexa Fluor® 488 anti-mouse TER-119/Erythroid -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3206	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Day-14 fetal liver cells from a C57BL/6 mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting¹, complement-mediated cytotoxicity³, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References (PubMed link indicates BioLegend citation)

Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
Vannucchi AM, et al. 2000. Blood 95:2559.
Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
Grisendi S, et al. 2005. Nature 437:147. (FC)
Bourdeau A, et al. 2007. Blood 109:4220.
Chappaz S, et al. 2007. Blood 110:3862. (FC)
Heuser M, et al. 2007. Blood 110:1639. (FC)
Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed

Product Citations

Santos J, et al. 2015. Sci Rep. 5: 16034. PubMed
Meissburger B, et al. 2016. Biochim Biophys Acta. 1861: 1121-1131. PubMed
Kehrer J, et al. 2016. PLoS Pathog. 12: 1005734. PubMed
Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
Yang X, et al. 2020. Blood Sci. 2:89. PubMed
Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
Yip HYK, et al. 2021. STAR Protocols. 2(3):100765. PubMed
Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
Wang X, et al. 2021. Front Cardiovasc Med. 8:642751. PubMed
Yip HYK, et al. 2020. Molecular Cell. 80(2):279-295.e8. PubMed
Kehrer J, Frischknecht F, Mair G 2016. Mol Cell Proteomics. 15: 2852 - 2862. PubMed
Hu M, et al. 2020. Cancer Immunol Res. 8:1150. PubMed
Tojo Y, et al. 2015. Mol Cell Biol. 35: 2658-2672. PubMed

RRID

AB_493402 (BioLegend Cat. No. 116215)

Antigen Details

Structure: Associated with glycophorin A, 52 kD
Distribution: Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E
Cell Type: Erythrocytes
Biology Area: Immunology
Antigen References: 1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.
Gene ID: 104231 View all products for this Gene ID
UniProt: View information about TER-119 on UniProt.org

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