Purified anti-Clathrin Heavy Chain (CLTCL2) Antibo
- 产品名称:
- Purified anti-Clathrin Heavy Chain (CLTCL2) Antibo
- 产品类别:
- 抗体
- 产品编号:
- 813901
- 产品应用:
- 813901
- Verified Reactivity
- Human, Drosophila
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised using the globular N-terminal domain of clathrin heavy chain.
- Formulation
- Phosphate-buffered solution + 0.03% Thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
WB - Quality tested
ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 ?g per mL.?For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL?is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody reacts with clathrin heavy chains (CHC). The antibody is useful for immunoblotting of clathrin heavy chains from cow CHC and immunofluorescence. It does not react with native clathrin in immunoprecipitation.
Reactivity to Drosophila was only verified with the purified format.
- Additional Product Notes
For Western blotting, the suggested use of this reagent is 5 -10 ?g/ml in human, mouse, and rat and 1-10 ?g/ml in Drosophila.
- Application References
(PubMed link indicates BioLegend citation) -
- Hood FE and Royle SJ. 2009. J Cell Sci. 122:2185-2190. (ICC) PubMed
- Jiang R, et al. 2000. J Biol Chem 275(12):8439-8447. (IP)
- Product Citations
-
- Royle F 2009. J Cell Sci. 122:2185-2190. PubMed
- Martínez–Fábregas J, et al. 2019. Elife. 8:e49314. PubMed
- RRID
- AB_2564779 (BioLegend Cat. No. 813901)