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Purified anti-Clathrin Heavy Chain (CLTCL2) Antibo
产品名称:
Purified anti-Clathrin Heavy Chain (CLTCL2) Antibo
产品类别:
抗体
产品编号:
813901
产品应用:
813901
[价格]
规格 价格 库存
100µL ¥ 4032 1

产品详情

Product Details

Verified Reactivity
Human, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised using the globular N-terminal domain of clathrin heavy chain.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 ?g per mL.?For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL?is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody reacts with clathrin heavy chains (CHC). The antibody is useful for immunoblotting of clathrin heavy chains from cow CHC and immunofluorescence. It does not react with native clathrin in immunoprecipitation.

Reactivity to Drosophila was only verified with the purified format.

Additional Product Notes

For Western blotting, the suggested use of this reagent is 5 -10 ?g/ml in human, mouse, and rat and 1-10 ?g/ml in Drosophila.

Application References

(PubMed link indicates BioLegend citation)
  1. Hood FE and Royle SJ. 2009. J Cell Sci. 122:2185-2190. (ICC) PubMed
  2. Jiang R, et al. 2000. J Biol Chem 275(12):8439-8447. (IP)
Product Citations
  1. Royle F 2009. J Cell Sci. 122:2185-2190. PubMed
  2. Martínez–Fábregas J, et al. 2019. Elife. 8:e49314. PubMed
RRID
AB_2564779 (BioLegend Cat. No. 813901)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID
1213 View all products for this Gene ID
UniProt
View information about Clathrin Heavy Chain on UniProt.org

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