PerCP/Cyanine5.5 anti-mouse CD150 (SLAM) Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD150 (SLAM) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 115921
- 产品应用：
- 115921
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse SLAM-human IgG1 fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion17, enhancing IFN-? production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 115949 & 115950).
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Castro AG, et al. 1999. J. Immunol. 163:5860. (FC, Costim, IP)
- Forsberg EC, et al. 2005. PLoS Genet. 1:e28. (FC)
- Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (FC)
- Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC)
- Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC)
- Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC, Block) PubMed
- Jung Y, et al. 2007. Blood 110:82. PubMed
- Pimanda JE, et al. 2007. Proc. Natl. Acad. Sci. USA 104:840.
- Sugiyama T, et al. 2007. Proc. Natl. Acad. Sci. USA 104:175.
- Kim I, et al. 2006. Blood 108:737. PubMed
- Ema H, et al. 2006. Nat Protoc. 1:2979. PubMed
- Fraser ST, et al. 2007. Blood 109:4616. PubMed
- Jung Y, et al. 2008. Stem Cells. 26:2042. Pubmed
- Song J, et al. 2010. Blood 115:2592. PubMed
- Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 43:149. (FC) PubMed
- Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
- Talaei N, et al. 2015. J. Immunol. 195(10):4623. PubMed
- Product Citations
-
- Abraham A, et al. 2019. J Clin Invest. 130:2685. PubMed
- Bae J, et al. 2019. Nat Commun. 10:3496. PubMed
- Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
- Zhang B, et al. 2018. Nat Med. 24:450. PubMed
- Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
- Mitroulis I et al. 2018. Cell. 172(1-2):147-161 . PubMed
- Guo B, et al. 2018. Nat Med. 24:360. PubMed
- Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
- Wu HC, et al. 2021. Cancer Discov. Online ahead of print. PubMed
- Simon M, et al. 2019. Cell Metab. 29:871. PubMed
- Yoshida K, et al. 2022. Sci Rep. 12:17276. PubMed
- Ruppert R, et al. 2015. J Exp Med. 212: 1415-1432. PubMed
- McAlpine CS, et al. 2021. Nature. 595:701. PubMed
- Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
- Georgievski A, et al. 2022. Cell Death Dis. 13:337. PubMed
- Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
- Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
- Yuzugullu H, et al. 2015. Nat Commun. 6: 8501. PubMed
- Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
- Agarwal P, et al. 2019. Cell Stem Cell. 24:769. PubMed
- Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
- Morganti C, et al. 2022. EMBO Rep. 23:e54262. PubMed
- Xiong X, et al. 2014. Elife. 21:3. PubMed
- Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
- Agarwal P, et al. 2021. Cell Reports. 36(2):109386. PubMed
- Guarnerio J, et al. 2018. Nat Commun. 9:66. PubMed
- RRID
- AB_2206887 (BioLegend Cat. No. 115921) AB_2303663 (BioLegend Cat. No. 115922)
- Structure
- Ig superfamily, 75-95 kD
- Distribution
-
Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells
- Function
- B cell and dendritic cell costimulation
- Ligand/Receptor
- CD150
- Cell Type
- B cells, Dendritic cells, Endothelial cells, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614.
- Gene ID
- 27218 View all products for this Gene ID
- UniProt
- View information about CD150 on UniProt.org
