PE/Cyanine7 anti-mouse CD150 (SLAM) Antibody
- 产品名称:
- PE/Cyanine7 anti-mouse CD150 (SLAM) Antibody
- 产品类别:
- 抗体
- 产品编号:
- 115913
- 产品应用:
- 115913
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse SLAM-human IgG1 fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The CD150 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion17, enhancing IFN-? production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 115949 & 115950).
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Castro AG, et al. 1999. J. Immunol. 163:5860. (FC, Costim, IP)
- Forsberg EC, et al. 2005. PLoS Genet. 1:e28. (FC)
- Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (FC)
- Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC)
- Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC)
- Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC, Block) PubMed
- Jung Y, et al. 2007. Blood 110:82. PubMed
- Pimanda JE, et al. 2007. Proc. Natl. Acad. Sci. USA 104:840.
- Sugiyama T, et al. 2007. Proc. Natl. Acad. Sci. USA 104:175.
- Kim I, et al. 2006. Blood 108:737. PubMed
- Ema H, et al. 2006. Nat Protoc. 1:2979. PubMed
- Fraser ST, et al. 2007. Blood 109:4616. PubMed
- Jung Y, et al. 2008. Stem Cells. 26:2042. Pubmed
- Song J, et al. 2010. Blood 115:2592. PubMed
- Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 43:149. (FC) PubMed
- Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
- Talaei N, et al. 2015. J. Immunol. 195(10):4623. PubMed
- Product Citations
-
- Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
- Upadhaya S, et al. 2020. Cell Stem Cell. 27(2):336-345.e4. PubMed
- Luchsinger LL, et al. 2020. Cell Stem Cell. 25(2):225-240. PubMed
- Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
- Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
- Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
- Muto T, et al. 2020. Nat Immunol. 1.246527778. PubMed
- Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
- Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
- Groenen AG, et al. 2022. J Lipid Res. 63:100167. PubMed
- Grinenko T, et al. 2018. Nat Commun. 9:1898. PubMed
- Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
- Pack AD, et al. 2021. Cell Reports. 36(8):109586. PubMed
- Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
- Sykes DB et al. 2016. Cell. 167(1):171-186 . PubMed
- Bonnet C, et al. 2021. iScience. 24:103399. PubMed
- Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
- Wilkinson AC, et al. 2020. Nature Protocols. 15(2):628-648.. PubMed
- Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
- Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
- Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
- Jordan M, et al. 2011. J Immunol. 186:3953. PubMed
- Eisele AS, et al. 2022. Elife. 11:. PubMed
- Gustafsson K, et al. 2013. Exp Cell Res. 319:1852. PubMed
- Halene S, et al. 2010. Blood. 116:1942. PubMed
- Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
- Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
- Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
- Mansell E, et al. 2020. Cell Stem Cell. . PubMed
- Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
- Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
- de Almeida MJ et al. 2017. Cell stem cell. 21(6):725-729 . PubMed
- Zhong L, et al. 2022. JCI Insight. 7:. PubMed
- Clapes T, et al. 2021. Nat Cell Biol. 23:704. PubMed
- Yankova E, et al. 2021. Nature. 593:597. PubMed
- Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed
- Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
- Passaro D et al. 2017. Cancer cell. 32(3):324-341 . PubMed
- Maetzig T, et al. 2017. Mol Ther. 25:606. PubMed
- Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
- Tober J, et al. 2013. Development. 140:3765. PubMed
- Yang T, et al. 2022. Nat Commun. 13:4464. PubMed
- Tummers B, et al. 2020. Immunity. 52(6):994-1006.e8. PubMed
- Liang R, et al. 2020. Cell Stem Cell. 359:26. PubMed
- Yu W, et al. 2020. J Clin Invest. . PubMed
- Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
- Hormaechea-Agulla D, et al. 2021. Cell Stem Cell. . PubMed
- Karamitros D, et al. 2015. Development. 142:70. PubMed
- de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
- Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
- Florez MA, et al. 2020. Cell Rep. 33:108530. PubMed
- Xia P, et al. 2015. J Exp Med. 212: 2305 - 2321. PubMed
- Nestorowa S, et al. 2016. Blood. 128: e20 - e31. PubMed
- Tzelepis K, et al. 2018. Nat Commun. 9:5378. PubMed
- Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
- Glotzbach J, et al. 2011. PLoS One. 6:e21211. PubMed
- Young IC, et al. 2021. Cell Rep. 37:110036. PubMed
- Koide S, et al. 2022. iScience. 25:103603. PubMed
- van den Berk P, et al. 2020. Cell Rep. 33:108533. PubMed
- Ueda K, et al. 2021. Cancer Cell. 39(4):529-547.e7. PubMed
- Chlon TM, et al. 2021. Cell Stem Cell. :. PubMed
- Viaud M, et al. 2020. Cell Reports. 30(10):3397-3410. PubMed
- Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
- Kent D, et al. 2009. Blood. 113:6342. PubMed
- Wilkinson AC, et al. 2019. Nature. 571:117. PubMed
- Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
- Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
- Luo H, et al. 2019. Cell Rep. 26:945. PubMed
- Guo F, et al. 2013. Haematologica. 98:1353. PubMed
- Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed
- Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
- Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
- Fast EM, et al. 2021. Elife. 10:. PubMed
- Lv K, et al. 2021. Cell Stem Cell. 28(7):1275-1290.e9. PubMed
- Rimmelé P, et al. 2015. EMBO Rep. 16: 1164-1176. PubMed
- Gerhardt D, et al. 2014. Genes Dev. 28:576. PubMed
- RRID
- AB_439796 (BioLegend Cat. No. 115913) AB_439797 (BioLegend Cat. No. 115914)
- Structure
- Ig superfamily, 75-95 kD
- Distribution
-
Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells
- Function
- B cell and dendritic cell costimulation
- Ligand/Receptor
- CD150
- Cell Type
- B cells, Dendritic cells, Endothelial cells, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614.
- Gene ID
- 27218 View all products for this Gene ID
- UniProt
- View information about CD150 on UniProt.org