PE/Cyanine5 anti-mouse CD150 (SLAM) Antibody
- 产品名称：
- PE/Cyanine5 anti-mouse CD150 (SLAM) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 115911
- 产品应用：
- 115911
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse SLAM-human IgG1 fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion17, enhancing IFN-? production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 115949 & 115950).
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Castro AG, et al. 1999. J. Immunol. 163:5860. (FC, Costim, IP)
- Forsberg EC, et al. 2005. PLoS Genet. 1:e28. (FC)
- Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (FC)
- Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC)
- Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC)
- Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC, Block) PubMed
- Jung Y, et al. 2007. Blood 110:82. PubMed
- Pimanda JE, et al. 2007. Proc. Natl. Acad. Sci. USA 104:840.
- Sugiyama T, et al. 2007. Proc. Natl. Acad. Sci. USA 104:175.
- Kim I, et al. 2006. Blood 108:737. PubMed
- Ema H, et al. 2006. Nat Protoc. 1:2979. PubMed
- Fraser ST, et al. 2007. Blood 109:4616. PubMed
- Jung Y, et al. 2008. Stem Cells. 26:2042. Pubmed
- Song J, et al. 2010. Blood 115:2592. PubMed
- Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 43:149. (FC) PubMed
- Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
- Talaei N, et al. 2015. J. Immunol. 195(10):4623. PubMed
- Product Citations
-
- Bowling S, et al. 2020. Cell. 181(6):1410-1422.e27. PubMed
- Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
- Sch?nberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
- Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
- Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
- He S, et al. 2011. Genes Dev. 25:1613. PubMed
- Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
- Cabezas–Wallscheid N, et al. 2017. Cell. 169:807. PubMed
- Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed
- Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
- Yu VWC, et al. 2017. Cell. 168:944. PubMed
- Thoms J, et al. 2016. Mol Cell Biol. 36: 1222-1236. PubMed
- Giannini S, et al. 2020. Nat Commun. 0.705555556. PubMed
- Austin W, et al. 2012. J Exp Med. 209:2215. PubMed
- Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
- Cheng E, et al. 2009. Blood. 113:2826. PubMed
- Sommerkamp P, et al. 2020. Cell Stem Cell. 26(5):722-738. PubMed
- Young K, et al. 2021. Cell Stem Cell. . PubMed
- RRID
- AB_493599 (BioLegend Cat. No. 115911) AB_493598 (BioLegend Cat. No. 115912)
- Structure
- Ig superfamily, 75-95 kD
- Distribution
-
Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells
- Function
- B cell and dendritic cell costimulation
- Ligand/Receptor
- CD150
- Cell Type
- B cells, Dendritic cells, Endothelial cells, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614.
- Gene ID
- 27218 View all products for this Gene ID
- UniProt
- View information about CD150 on UniProt.org
