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Brilliant Violet 605™ anti-mouse CD150 (SLAM
产品名称:
Brilliant Violet 605™ anti-mouse CD150 (SLAM
产品类别:
抗体
产品编号:
115927
产品应用:
115927
[价格]
规格 价格 库存
125µL ¥ 3234 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse SLAM-human IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605? excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion17, enhancing IFN-? production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 115949 & 115950).

Application References

(PubMed link indicates BioLegend citation)
  1. Castro AG, et al. 1999. J. Immunol. 163:5860. (FC, Costim, IP)
  2. Forsberg EC, et al. 2005. PLoS Genet. 1:e28. (FC)
  3. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (FC)
  4. Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC)
  5. Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC)
  6. Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC, Block) PubMed
  7. Jung Y, et al. 2007. Blood 110:82. PubMed
  8. Pimanda JE, et al. 2007. Proc. Natl. Acad. Sci. USA 104:840.
  9. Sugiyama T, et al. 2007. Proc. Natl. Acad. Sci. USA 104:175.
  10. Kim I, et al. 2006. Blood 108:737. PubMed
  11. Ema H, et al. 2006. Nat Protoc. 1:2979. PubMed
  12. Fraser ST, et al. 2007. Blood 109:4616. PubMed
  13. Jung Y, et al. 2008. Stem Cells. 26:2042. Pubmed
  14. Song J, et al. 2010. Blood 115:2592. PubMed
  15. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 43:149. (FC) PubMed
  16. Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
  17. Talaei N, et al. 2015. J. Immunol. 195(10):4623. PubMed
Product Citations
  1. Ho YH, et al. 2019. Cell Stem Cell. 25:407. PubMed
  2. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  3. Sch?nberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  4. Forte D, et al. 2020. Cell Metab. 32:829. PubMed
  5. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  6. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  7. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  8. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  9. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
  10. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  11. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  12. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  13. Brandi P, et al. 2022. Cell Rep. 38:110184. PubMed
  14. Zhang Z, et al. 2019. Haematologica. 4.339583333. PubMed
  15. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  16. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  17. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  18. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  19. Sch?nberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  20. Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed
  21. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  22. Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
  23. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
  24. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  25. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
RRID
AB_11204248 (BioLegend Cat. No. 115927)

Antigen Details

Structure
Ig superfamily, 75-95 kD
Distribution

Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells

Function
B cell and dendritic cell costimulation
Ligand/Receptor
CD150
Cell Type
B cells, Dendritic cells, Endothelial cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614.

Gene ID
27218 View all products for this Gene ID
UniProt
View information about CD150 on UniProt.org

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