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首页 > 抗体蛋白 > 抗体
Biotin anti-mouse IL-17A Antibody
产品名称：
Biotin anti-mouse IL-17A Antibody
产品类别：
抗体
产品编号：
507001
产品应用：
507001
[价格]
规格 价格 库存
50µg ￥ 1652 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection, ELISPOT Detection
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-17 recombinant protein ranging from 1000 to 15.63 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA2 and ELISPOT3 Detection: The biotinylated TC11-8H4 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified TC11-18H10 antibody (Cat. No. 506901) as the capture antibody, and with recombinant mouse IL-17 (Cat. No. 564101) as the standard.

Application References

(PubMed link indicates BioLegend citation)
  1. Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
  2. Herrada A, et al. 2010. J. Immunol. 184:191
  3. Nekrasova T, et al. 2005. J. Immunol. 175:2734.
Product Citations
  1. Haas K, et al. 2014. J Infect Dis. 209:87. PubMed
  2. Ji N, et al. 2015. Neurol Neuroimmunol Neuroinflamm. 2: e139. PubMed
  3. Honda-Okubo Y, et al. 2015. J Virol. 89:2995. PubMed
  4. Yu H, et al. 2010. Infect Immun. 78:2272. PubMed
  5. Sosa R, et al. 2013. J Immunol. 191:5848. PubMed
  6. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
  7. Sosa R, et al. 2015. Proc Natl Acad Sci U S A. 112: 5038-5047. PubMed
RRID
AB_315465 (BioLegend Cat. No. 507001) AB_315466 (BioLegend Cat. No. 507002)

Antigen Details

Structure
Cytokine; dimer; 15 kD (Mammalian)
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
CD4+ memory T cells
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R (CD217)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006 Nat. Rev. Immunol. 6(4):329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237(2):123.

Gene ID
16171 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

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