Ultra-LEAF™ Purified anti-mouse IL-17A Antib-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2548	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli expressed, recombinant mouse IL-17A

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
CyTOF? - Verified
ELISPOT Capture, ICFC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture, a concentration range of 2-6 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-17 recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture^3,4 and ELISPOT Capture⁵: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry^{2-4,7,8,11,12}: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization^6,9: The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).

Application References (PubMed link indicates BioLegend citation)

Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
Schubert D, et al. 2004. J. Immunol. 172:4503. (ICFC)
Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (ICFC, ELISA Capture)
Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (ICFC, ELISA Capture)
Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture)
Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut)
Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (ICFC)
Kang SG, et al. 2007. J. Immunol. 179:3724. (ICFC)
Smith E, et al. 2008. J. Immunol. 181:1357. (Neut) PubMed
Neufert C, et al. 2007. Eur. J. Immunol. 37:1809. PubMed
Wang C, et al. 2009. Mucosal Immunol 2:173. (ICFC) PubMed
Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (ICFC) PubMed
Kivis?kk P, et al. 2009. Ann. Neurol. 65:457. PubMed
Cooney LA, et al. 2011. J. Immunol. 187:4440. PubMed
Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
Murakami R, et al. 2013. PLoS One. 8:73270. PubMed

Product Citations

Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed

RRID

AB_2783334 (BioLegend Cat. No. 506945) AB_2783335 (BioLegend Cat. No. 506946)

Antigen Details

Structure: Cytokine; dimer; 15 kD (Mammalian).
Bioactivity: Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources: CD4⁺ memory T cells
Cell Targets: Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors: IL-17R (CD217)
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.
Gene ID: 16171 View all products for this Gene ID
UniProt: View information about IL-17A on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们