Purified anti-mouse IL-17A (Maxpar® Ready) Ant-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2394	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli expressed, recombinant mouse IL-17A

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

ELISA Capture^3,4 and ELISPOT Capture⁵: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry^{2-4,7,8,11,12}: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization^6,9: The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
Schubert D, et al. 2004. J. Immunol. 172:4503. (ICFC)
Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (ICFC, ELISA Capture)
Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (ICFC, ELISA Capture)
Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture)
Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut)
Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (ICFC)
Kang SG, et al. 2007. J. Immunol. 179:3724. (ICFC)
Smith E, et al. 2008. J. Immunol. 181:1357. (Neut) PubMed
Neufert C, et al. 2007. Eur. J. Immunol. 37:1809. PubMed
Wang C, et al. 2009. Mucosal Immunol 2:173. (ICFC) PubMed
Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (ICFC) PubMed
Kivis?kk P, et al. 2009. Ann. Neurol. 65:457. PubMed
Cooney LA, et al. 2011. J. Immunol. 187:4440. PubMed
Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
Murakami R, et al. 2013. PLoS One. 8:73270. PubMed

Product Citations

McDonald B, et al. 2020. Cell Host Microbe. 28(5):660-668.e4. PubMed

RRID

AB_2562850 (BioLegend Cat. No. 506935)

Antigen Details

Structure: Cytokine; dimer; 15 kD (Mammalian).
Bioactivity: Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources: CD4⁺ memory T cells
Cell Targets: Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors: IL-17R (CD217)
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.
Gene ID: 16171 View all products for this Gene ID
UniProt: View information about IL-17A on UniProt.org

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